Figure 2

Effect of NO on MAdCAM-1 in SVEC. Cells were pretreated with (A) DETA-NONOate or (B) Spermine-NONOate (SNO) with indicated concentrations and then exposed to TNF-α (20 ng/ml) stimulation in the continued presence of NO donors. MAdCAM-1 expression is significantly increased after TNF-α. DETA-NONOate or Spermine-NONOate (SNO) blocked the TNF-α induced MAdCAM-1 expression dose dependently. Values represent mean ± SE; each group (n = 3). ^* p < 0.001 vs. untreated controls, ^# p < 0.001 vs. TNF-α treatment. (C) Effect of NO on p65 NF-κB translocation. Immunoblots of NF-κB p65 in nuclear extracts from SVEC. Nuclear extracts (40 μg) were loaded in each well. (B) Inhibition of TNF-α activation of NF-κB in SVEC. After 1 h of TNF-α (20 ng/ml) treatment nuclear p65 of NF-κB is induced. Pretreatment of the cells with DETA-NONOate (100 μM) or Spermine-NONOate (100 μM) reduced the TNF-α induced nuclear translocation of the NF-κB p65 subunit.