Symptoms and signs in individuals with serology positive for celiac disease but normal mucosa
© Ludvigsson et al; licensee BioMed Central Ltd. 2009
Received: 01 April 2009
Accepted: 22 July 2009
Published: 22 July 2009
Antibody serology is an important tool in the investigation of celiac disease (CD), but does not always correlate with mucosal appearance in the small intestine. Patients with positive CD serology but normal mucosa (Marsh 0) are at increased risk of future CD. In this study we describe a model for identifying and characterizing individuals with normal mucosa but positive CD serology. Such individuals are sometimes referred to as having latent CD.
The records of ten Swedish pathology departments were used to identify individuals with biopsies indicating normal duodenal/jejunal mucosa. Using the national personal identification number, these data were linked with CD serology data (antigliadin, antiendomysial and tissue transglutaminase antibodies); and we thereby identified 3,736 individuals with normal mucosa but positive CD serology. Two independent reviewers then manually reviewed their biopsy reports to estimate comorbidity. We also randomly selected 112 individuals for validation through patient chart review.
The majority of the 3,736 individuals were females (62%). Children (0–15 years) made up 21.4%. The median number of biopsy specimen was 3. Our review of biopsy reports found that other gastrointestinal comorbidity was rare (inflammatory bowel disease: 0.4%; helicobacter pylori infection: 0.2%). Some 22% individuals selected for patient chart review had a relative with CD. The most common symptoms among these individuals were diarrhea (46%) and abdominal pain (45%), while 26% had anemia. Although 27% of the individuals selected for validation had been informed about gluten-free diet, only 13% were adhering to a gluten-free diet at the end of follow-up.
Individuals with positive CD serology but normal mucosa often have CD-like symptoms and a family history of CD.
Celiac disease (CD) is an immune-mediated disorder usually confirmed through small-intestinal biopsy[1, 2]. In the last 10–15 years, the use of CD serology (antigliadin antibodies (AGA), antiendomysial antibodies (EMA)[4, 5], and tissue transglutaminase antibodies (TTGA) [6–8]) has changed the diagnostic algorithm for CD . At first, only AGA was used, but recently, this antibody has been largely replaced by EMA and TTGA except in young children. We have earlier shown that 68/68 (100%) Swedish pediatricians and 141/180 (78%) adult gastroenterologist use CD serology in their work-up for CD in at least 90% of individuals with suspected CD. More than 95% of Swedish pediatricians and adult gastroenterologists perform a small intestinal biopsy prior to a diagnosis of CD .
In individuals with CD, the biopsy typically shows villous atrophy (VA), crypt hyperplasia and inflammation [12, 13]. However, some individuals with positive CD serology have a normal mucosa (Marsh 0). Many of those later develop CD [14–17], and the term "latent CD" is sometimes used when referring to such individuals .
We linked Swedish biopsy registers with biochemistry registers to identify individuals with positive CD serology but normal small intestinal mucosa. The purposes of this paper were to (I) describe a model for identifying such patients, (II) describe the symptoms and signs in a subset of patients, and to (III) evaluate if registry-matching is an effective means to identify individuals with positive CD serology but normal small intestinal mucosa.
Patients were identified through matching of normal biopsy data (obtained from pathology departments) and data on positive CD serology (obtained from biochemistry departments).
Classification of biopsy data
To exclude a diagnosis of CD, Swedish pathologists will examine the crypt-villous ratio, the different layers of the intestine, and the number of intraepithelial lymphocytes in small intestinal biopsies. All Swedish pathology departments also use CD3 immunostaining to detect intraepithelial lymphocytosis  (classified as inflammation (Marsh 1–2) in Sweden). For a description of the Swedish SnoMed system, and a complete listing of histopathology codes used in this study please see Additional File 1. In the current study, we defined normal mucosa (Marsh 0)  as either of the SnoMed codes, M0010 and M0011.
Collection of biopsy data
Misclassification of individuals identified through registry matching
From among the 3,736 individuals, we randomly selected 120 identified through registry matching (EMA+: n = 40; AGA+: n = 40; TTGA+: n = 40), and requested their patient charts from the department or health care centre that requested the CD serology test, as well as from the department performing the small intestinal biopsy (usually departments of internal medicine/gastroenterology, surgery or pediatrics).
Using individual medical records (and when missing through phone contact with the responsible department/physician), we assessed the proportion of individuals who were correctly identified as having a macroscopically normal mucosa but positive CD serology, and who had never had a biopsy with inflammation or VA).
Again using medical records, we then examined symptoms, signs, laboratory measures, and the extent to which individuals had received information about gluten-free diet. This information was divided according to type of CD serology, and is presented both summarized and weighted according to the distribution of EMA+, TTGA+ and AGA+ among the 3,736 individuals from the complete data-set.
Comorbidity in biopsy samples
In order to determine if the positive CD serology could be due to other concomitant gastrointestinal disorder (other than pre-stage CD) or systemic disorders with gastrointestinal involvement, we performed a computerized search of the text of the 3,736 biopsy reports with normal mucosa but positive CD serology (see also our earlier paper on VA and inflammation ). Thereby we identified 67 biopsy reports where there were indications of comorbidity other than CD. Each of these biopsy reports was then manually screened by two independent reviewers (JFL and research assistant) to confirm or reject the presence of comorbidity. Discrepancies were resolved through a third review of the biopsy reports where necessary.
Quality assurance and accreditation of CD serology
To explore the quality of CD serology in Sweden we obtained data from the organization Equalis, which has provided laboratory quality assurance in Sweden since 1999 http://www.equalis.se/. The quality assurance scheme can be described briefly as follows. Each year, one of the participating laboratories submits eight blinded CD serology samples to Equalis and a short accompanying clinical description of the individual. From among these eight samples (some positive, some negative for CD serology), Equalis distributes two samples to all participating laboratories for analysis and test data are then reported to Equalis. All assurance data are then merged and presented in aggregated form.
Equalis also provides accreditation of CD serology, and we estimated the proportion of CD serology samples that were accredited and originated from individuals with a small intestinal biopsy (with normal mucosa, inflammation, or VA) in any of the ten participating pathology departments. We then estimated the proportion of accredited CD serology samples from any individual undergoing biopsy in the ascertainment area of these biochemistry departments (independent of the histopathology of the patient).
We calculated 95% confidence intervals (CI). In post-hoc analyses we compared the prevalence of symptoms and signs according to the type of CD serology (EMA vs. AGA vs. TTGA) using the Chi-2 test (Kruskal-Wallis test was used when we examined year of biopsy and age at first biopsy). To decrease the risk of false-positive statistical significance due to multiple testing we used the Bonferroni correction when comparing the patient characteristics according to CD serology subtype ; and statistical significance level was set to <0.003 (0.05/18 comparisons) .
The current study was performed as part of a larger project on complications in CD. That study was approved by the Regional Ethical Review Board in Stockholm on the 4th of June 2006 (2006/633-31/4) with additional amendments (2007/747-32 and 2008/257-32).
Characteristics of individuals with latent celiac disease
Age, yrs (median, range)
Children = 15 years (%)
Children = 21 years (%)
Entry year (median, range)
Clinical characteristics of latent CD – patient chart review.
N = 38
N = 36
N = 38
N = 112
N = 3,736
Age at first biopsy: median, range (years)
Year of biopsy, median*
Reported heredity for CD
Diabetes Mellitus, type 1
Liver disease or increased liver enzymes
Any gastrointestinal symptom#
Weight loss/growth failure
Anemia and/or iron-deficiency
Folic acid deficiency
Erythrocyte sedimentation rate, increased
Misclassification of individuals identified through registry matching (n = 114)
The mean number of biopsy specimens at the first negative biopsy was 2.8 (median = 3). Since several individuals were biopsied on more than one occasion, the estimated mean number of biopsy specimens with normal mucosa collected throughout the entire study period was 3.2.
Symptoms and signs in individuals with normal mucosa but positive CD serology (n = 112)
Diarrhea was seen in 46% of individuals (95% CI = 36–55%), and constipation in 18% (95% CI = 11–26%). Anemia occurred in 26% of the individuals (95% CI = 18–35%), while B12 deficiency occurred in one out of ten individuals (Table 2). Depression and liver disease were both seen in about 10% of individuals with normal mucosa but positive CD serology. Type 1 diabetes mellitus occurred in 8% of individuals and thyroid disease in 11%.
When we restricted our data to children (n = 39), diarrhea was seen in 49% (95% CI = 32–65%), weight loss/growth failure in 38% (95% CI = 23–55%), constipation in 28% (95% CI = 15–45%), and anemia in 18% (95% CI = 8–34%). There were no statistically significant differences in symptoms and signs in comparisons using the various measures of CD serology, EMA+, AGA+, TTGA+, (data not shown).
Information about gluten-free diet
Gluten-free diet in latent CD (%).
N = 38
N = 36
N = 38
N = 112
N = 3,736
Informed about gluten-free diet by physician or dietician
Clinical response to gluten-free diet*
Gluten-free diet at end of follow-up
However, only 13% (95% CI = 8–21%) were on a gluten-free diet at the end of follow-up of this study (year 2008).
Comorbidity in biopsy samples
Comorbidity in 67 biopsy samples – Results of manual examination
Helicobacter pylori infection
Accreditation of CD serology
CD serology – Accreditation of laboratories.
Number of serology tests
Karolinska Solna and Huddinge§
Sahlgrenska and Östra Hospitals§
Lund and Malmö Hospitals§
Through biochemistry registers and biopsy registers we were able to identify 3,736 individuals with normal mucosa but positive CD serology. Such individuals are at increased risk of future CD[15, 21]. Our patient chart review found that more than 90% of individuals identified through matching of biopsy registers and biochemistry registers were correctly identified. Previous data suggest that normal mucosa (Marsh 0) is very seldom misclassified (in a blinded test, 96% of samples with normal mucosa were correctly classified by Swedish pathologists ). In addition all surveyed Swedish pathology departments use CD3-immunostaining to detect intraepithelial lymphocytes. Most Swedish pathology departments consider >30 intraepithelial lymphocytes per 100 as abnormal (and not >40 as was suggested in older literature). Recent data indicate that a cut-off of 25 per 100 or 30 per 100 is appropriate .
The symptoms and signs of the 112 individuals whose patient charts were reviewed in this study were similar to those seen in CD [11, 24, 25]. This is however unsurprising, since most individuals in our cohort had probably been tested for CD serology and undergone small intestinal biopsy due to suspected CD. Earlier studies have found a varying prevalence of diarrhea in individuals with CD (Green: 85%; Ciacci 60%; Ludvigsson (children) 53%; Fasano 35%). This compares with 45% in the current study of individuals with a normal mucosa.
Some 85% of individuals in this study reported at least some kind of gastrointestinal symptoms. This was slightly higher proportion than in the study by Salmi et al (18/25 individuals (72%) with positive CD serology and Marsh 0–1 in their study had "abdominal symptoms"), but then we also included weight loss in our definition of gastrointestinal symptoms. The prevalence of constipation in the current study was almost identical to that of individuals with screening-detected CD in the Fasano et al study . In a British study, 50% of individuals with undiagnosed CD had anemia on presentation , and this may partly be due to occult gastrointestinal bleeding . Occult bleeding is however related to the degree of mucosal injury, and that may explain the lower rate of anemia in our population since they all had Marsh 0. Of our individuals, one in four had anemia and/or iron deficiency. Of note, we found no difference in the prevalence of symptoms and signs according to the type of CD serology (EMA+, AGA+, TTGA+).
Twenty-two percent of validated individuals had a family history of CD. This is consistent with data from an American study of adults with diagnosed CD , where 19% reported having a relative with CD. There are several studies suggesting that individuals with CD are at increased risk of having a relative with a diagnosis of CD [28, 31, 32], but to our knowledge ours is the first study to show this in individuals with normal mucosa but positive CD serology.
The specificity of CD serology is high , and a negative CD serology will almost always rule out a diagnosis of CD . However the positive predictive specificity (PPV) of CD serology is not infallible, with two recent papers showing a PPV for TTGA of 29%  and 76%  respectively. This does not mean that positive CD serology in individuals with normal mucosa is of no clinical significance. Many individuals with positive CD serology but normal mucosa will go on to develop VA [14–17]; and IgA AGA+, EMA+, and TTGA+ are all associated with increased mortality.
Due to the historical nature of this study, AGA was the only available screening tool for much of the study period (beginning in 1990). AGA has lower specificity for CD than TTGA and especially EMA. Although we cannot rule out the possibility that some individuals with positive CD serology and normal mucosa do not suffer from a raised risk for future CD it should be emphasized that the specificity of IgA AGA is nevertheless above 80% in adults  (around 90% in a later report by Rostom et al ). In children it may be even higher  (and more than 20% of our population consisted of children aged ≤15 years). These antibody tests were not performed as part of screening for CD in the general population but likely due to symptoms that also merited a small intestinal biopsy. Still, it should be remembered that not all individuals with positive CD serology develop CD , and that positive CD serology can sometimes be transient.
We chose to include individuals with positive IgG and normal mucosa in this study. IgA deficiency is strongly associated with CD [37–39], and in these individuals IgG is a frequently used screening tool for CD; and all individuals in our study had undergone small intestinal biopsy. Although IgG EMA and TTGA are associated with CD , less is known of the specificity and positive predictive value of IgG AGA.
Another potential limitation of our study is that some biopsy samples may have been misclassified. Small intestinal inflammation is sometimes missed by pathologists , and in patchy VA and inflammation, several biopsies are needed to confirm a CD diagnosis [42, 43]. Our study was based on historical data, when the use of capsule biopsy was widespread (with fewer biopsy specimen as a consequence), especially among pediatricians . On average, 3 biopsy specimens had been obtained from each individual, and some of the individuals may have had a false-negative patchy VA. Neither can we rule out that some individuals classified as having Marsh 0 had in fact sub-microscopic changes (microscopic enteritis), and we lack individual-based data on immunohistochemistry for our individuals [44, 45]. Microscopic enteritis has increasingly been recognized as an important cause of CD-like symptoms including malabsorption . These individuals have a low count of intraepithelial lymphocytes but nevertheless show altered enterocytes and affected microvilli . Microscopic enteritis is also associated with other autoimmune diseases.
Among individuals with normal mucosa but positive CD serology and available data, we also characterized the use of gluten-free diet. Approximately one individual in four had received information about gluten-free diet from a physician or from a dietician after having their biopsy. However, some individuals with positive CD serology may have had tried a gluten-free diet themselves, without prior prescription. Among individuals on a gluten-free diet, about half reported a clinical response. It has previously been shown that symptoms in individuals with minor mucosal abnormalities may improve on a gluten-free diet . This supports our belief that at least some individuals with normal mucosa but positive CD serology have early stage CD or a phenotype of CD.
Individuals with positive CD serology but normal mucosa often have CD-like symptoms and a family history of CD. Such individuals may be identified through registry matching.
A proportion of individuals with normal mucosa (Marsh 0) and positive CD serology may in fact have microscopic enteritis. Earlier research has shown that individuals with macroscopically normal mucosa but positive CD serology often progress to Marsh I-III with symptoms consistent with CD[15, 21].
Tissue transglutaminase antibodies
This project was supported by a grant from The Swedish Society of Medicine, the Örebro University Hospital, the Swedish Research Council, the Sven Jerring Foundation, the Örebro Society of Medicine, the Karolinska Institutet, the Clas Groschinsky Foundation, the Juhlin Foundation, the Majblomman Foundation and the Swedish Coeliac Society. We would like to thank the company Equalis for access to data on the accuracy of celiac disease serology.
- AGA Institute Medical Position Statement on the Diagnosis and Management of Celiac Disease. Gastroenterology. 2006, 131 (6): 1977-1980. 10.1053/j.gastro.2006.10.003.
- Rostom A, Murray JA, Kagnoff MF: American Gastroenterological Association (AGA) Institute technical review on the diagnosis and management of celiac disease. Gastroenterology. 2006, 131 (6): 1981-2002. 10.1053/j.gastro.2006.10.004.View ArticlePubMedGoogle Scholar
- Savilahti E, Viander M, Perkkio M, Vainio E, Kalimo K, Reunala T: IgA antigliadin antibodies: a marker of mucosal damage in childhood coeliac disease. Lancet. 1983, 1 (8320): 320-322. 10.1016/S0140-6736(83)91627-6.View ArticlePubMedGoogle Scholar
- Burgin-Wolff A, Gaze H, Hadziselimovic F, Huber H, Lentze MJ, Nussle D, Reymond-Berthet C: Antigliadin and antiendomysium antibody determination for coeliac disease [see comments]. Arch Dis Child. 1991, 66 (8): 941-947. 10.1136/adc.66.8.941.View ArticlePubMedPubMed CentralGoogle Scholar
- Ladinser B, Rossipal E, Pittschieler K: Endomysium antibodies in coeliac disease: an improved method. Gut. 1994, 35 (6): 776-778. 10.1136/gut.35.6.776.View ArticlePubMedPubMed CentralGoogle Scholar
- Dieterich W, Ehnis T, Bauer M, Donner P, Volta U, Riecken EO, Schuppan D: Identification of tissue transglutaminase as the autoantigen of celiac disease [see comments]. Nat Med. 1997, 3 (7): 797-801. 10.1038/nm0797-797.View ArticlePubMedGoogle Scholar
- Dieterich W, Laag E, Schopper H, Volta U, Ferguson A, Gillett H, Riecken EO, Schuppan D: Autoantibodies to tissue transglutaminase as predictors of celiac disease [see comments]. Gastroenterology. 1998, 115 (6): 1317-1321. 10.1016/S0016-5085(98)70007-1.View ArticlePubMedGoogle Scholar
- Sulkanen S, Halttunen T, Laurila K, Kolho KL, Korponay-Szabo IR, Sarnesto A, Savilahti E, Collin P, Maki M: Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease [see comments]. Gastroenterology. 1998, 115 (6): 1322-1328. 10.1016/S0016-5085(98)70008-3.View ArticlePubMedGoogle Scholar
- Hadithi M, von Blomberg BM, Crusius JB, Bloemena E, Kostense PJ, Meijer JW, Mulder CJ, Stehouwer CD, Pena AS: Accuracy of serologic tests and HLA-DQ typing for diagnosing celiac disease. Ann Intern Med. 2007, 147 (5): 294-302.View ArticlePubMedGoogle Scholar
- Hill ID: What are the sensitivity and specificity of serologic tests for celiac disease? Do sensitivity and specificity vary in different populations?. Gastroenterology. 2005, 128 (4 Suppl 1): S25-32. 10.1053/j.gastro.2005.02.012.View ArticlePubMedGoogle Scholar
- Ludvigsson JF, Brandt L, Montgomery SM, Granath F, Ekbom A: Validation study of villous atrophy and small intestinal inflammation in Swedish biopsy registers. BMC Gastroenterol. 2009, 9 (1): 19-10.1186/1471-230X-9-19.View ArticlePubMedPubMed CentralGoogle Scholar
- Dickson BC, Streutker CJ, Chetty R: Coeliac disease: an update for pathologists. J Clin Pathol. 2006, 59 (10): 1008-1016. 10.1136/jcp.2005.035345.View ArticlePubMedPubMed CentralGoogle Scholar
- Green PH, Cellier C: Celiac disease. N Engl J Med. 2007, 357 (17): 1731-1743. 10.1056/NEJMra071600.View ArticlePubMedGoogle Scholar
- Troncone R: Latent coeliac disease in Italy. The SIGEP Working Group on Latent Coeliac Disease. Italian Society for Paediatric Gastroenterology and Hepatology. Acta Paediatr. 1995, 84 (11): 1252-1257. 10.1111/j.1651-2227.1995.tb13543.x.View ArticlePubMedGoogle Scholar
- Collin P, Helin H, Maki M, Hallstrom O, Karvonen AL: Follow-up of patients positive in reticulin and gliadin antibody tests with normal small-bowel biopsy findings. Scand J Gastroenterol. 1993, 28 (7): 595-598. 10.3109/00365529309096094.View ArticlePubMedGoogle Scholar
- Salmi TT, Collin P, Jarvinen O, Haimila K, Partanen J, Laurila K, Korponay-Szabo IR, Huhtala H, Reunala T, Maki M, et al: Immunoglobulin A autoantibodies against transglutaminase 2 in the small intestinal mucosa predict forthcoming coeliac disease. Aliment Pharmacol Ther. 2006, 24 (3): 541-552. 10.1111/j.1365-2036.2006.02997.x.View ArticlePubMedGoogle Scholar
- Iltanen S, Holm K, Partanen J, Laippala P, Maki M: Increased density of jejunal gammadelta+ T cells in patients having normal mucosa – marker of operative autoimmune mechanisms?. Autoimmunity. 1999, 29 (3): 179-187.View ArticlePubMedGoogle Scholar
- National Institutes of Health Consensus Development Conference Statement on Celiac Disease, June 28–30 2004. Gastroenterology. 2005, 128 (4 Suppl 1): S1-9.
- Marsh MN: Grains of truth: evolutionary changes in small intestinal mucosa in response to environmental antigen challenge. Gut. 1990, 31 (1): 111-114. 10.1136/gut.31.1.111.View ArticlePubMedPubMed CentralGoogle Scholar
- Armitage P, Berry G, Matthews J: Statistical Methods in Medical Research. 2002, Oxford: Blackwell Science Ltd, 4View ArticleGoogle Scholar
- Troncone R, Greco L, Mayer M, Paparo F, Caputo N, Micillo M, Mugione P, Auricchio S: Latent and potential coeliac disease. Acta Paediatr Suppl. 1996, 412: 10-14. 10.1111/j.1651-2227.1996.tb14240.x.View ArticlePubMedGoogle Scholar
- Hayat M, Cairns A, Dixon MF, O'Mahony S: Quantitation of intraepithelial lymphocytes in human duodenum: what is normal?. J Clin Pathol. 2002, 55 (5): 393-394.View ArticlePubMedPubMed CentralGoogle Scholar
- Veress B, Franzen L, Bodin L, Borch K: Duodenal intraepithelial lymphocyte-count revisited. Scand J Gastroenterol. 2004, 39 (2): 138-144. 10.1080/00365520310007675.View ArticlePubMedGoogle Scholar
- Ciacci C, Cirillo M, Sollazzo R, Savino G, Sabbatini F, Mazzacca G: Gender and clinical presentation in adult celiac disease. Scand J Gastroenterol. 1995, 30 (11): 1077-1081. 10.3109/00365529509101610.View ArticlePubMedGoogle Scholar
- Dickey W, McConnell JB: How many hospital visits does it take before celiac sprue is diagnosed?. J Clin Gastroenterol. 1996, 23 (1): 21-23. 10.1097/00004836-199607000-00007.View ArticlePubMedGoogle Scholar
- Green PHR, Stavropoulos SN, Panagi SG, Goldstein SL, McMahon DJ, Absan H, Neugut AI: Characteristics of adult celiac disease in the USA: results of a national survey. Am J Gastroenterol. 2001, 96 (1): 126-131. 10.1111/j.1572-0241.2001.03462.x.View ArticlePubMedGoogle Scholar
- Ludvigsson JF, Ansved P, Falth-Magnusson K, Hammersjo JA, Johansson C, Edvardsson S, Ljungkrantz M, Stenhammar L, Ludvigsson J: Symptoms and Signs Have Changed in Swedish Children With Coeliac Disease. J Pediatr Gastroenterol Nutr. 2004, 38 (2): 181-186. 10.1097/00005176-200402000-00015.View ArticlePubMedGoogle Scholar
- Fasano A, Berti I, Gerarduzzi T, Not T, Colletti RB, Drago S, Elitsur Y, Green PH, Guandalini S, Hill ID, et al: Prevalence of celiac disease in at-risk and not-at-risk groups in the United States: a large multicenter study. Arch Intern Med. 2003, 163 (3): 286-292. 10.1001/archinte.163.3.286.View ArticlePubMedGoogle Scholar
- Hin H, Bird G, Fisher P, Mahy N, Jewell D: Coeliac disease in primary care: case finding study. Bmj. 1999, 318 (7177): 164-167.View ArticlePubMedPubMed CentralGoogle Scholar
- Fine KD: The prevalence of occult gastrointestinal bleeding in celiac sprue. N Engl J Med. 1996, 334 (18): 1163-1167. 10.1056/NEJM199605023341804.View ArticlePubMedGoogle Scholar
- Cataldo F, Marino V: Increased prevalence of autoimmune diseases in first-degree relatives of patients with celiac disease. J Pediatr Gastroenterol Nutr. 2003, 36 (4): 470-473. 10.1097/00005176-200304000-00009.View ArticlePubMedGoogle Scholar
- Neuhausen SL, Steele L, Ryan S, Mousavi M, Pinto M, Osann KE, Flodman P, Zone JJ: Co-occurrence of celiac disease and other autoimmune diseases in celiacs and their first-degree relatives. J Autoimmun. 2008, 31 (2): 160-165. 10.1016/j.jaut.2008.06.001.View ArticlePubMedPubMed CentralGoogle Scholar
- Hopper AD, Cross SS, Hurlstone DP, McAlindon ME, Lobo AJ, Hadjivassiliou M, Sloan ME, Dixon S, Sanders DS: Pre-endoscopy serological testing for coeliac disease: evaluation of a clinical decision tool. Bmj. 2007, 334 (7596): 729-10.1136/bmj.39133.668681.BE.View ArticlePubMedPubMed CentralGoogle Scholar
- Anderson LA, McMillan SA, Watson RG, Monaghan P, Gavin AT, Fox C, Murray LJ: Malignancy and mortality in a population-based cohort of patients with coeliac disease or "gluten sensitivity". World J Gastroenterol. 2007, 13 (1): 146-151.View ArticlePubMedPubMed CentralGoogle Scholar
- Metzger MH, Heier M, Maki M, Bravi E, Schneider A, Lowel H, Illig T, Schuppan D, Wichmann HE: Mortality excess in individuals with elevated IgA anti-transglutaminase antibodies: the KORA/MONICA Augsburg cohort study 1989–1998. Eur J Epidemiol. 2006, 21 (5): 359-365. 10.1007/s10654-006-9002-4.View ArticlePubMedGoogle Scholar
- Rostom A, Dube C, Cranney A, Saloojee N, Sy R, Garritty C, Sampson M, Zhang L, Yazdi F, Mamaladze V, et al: The diagnostic accuracy of serologic tests for celiac disease: a systematic review. Gastroenterology. 2005, 128 (4 Suppl 1): S38-46. 10.1053/j.gastro.2005.02.028.View ArticlePubMedGoogle Scholar
- Meini A, Pillan NM, Villanacci V, Monafo V, Ugazio AG, Plebani A: Prevalence and diagnosis of celiac disease in IgA-deficient children. Ann Allergy Asthma Immunol. 1996, 77 (4): 333-336.View ArticlePubMedGoogle Scholar
- Cataldo F, Marino V, Bottaro G, Greco P, Ventura A: Celiac disease and selective immunoglobulin A deficiency. J Pediatr. 1997, 131 (2): 306-308. 10.1016/S0022-3476(97)70172-0.View ArticlePubMedGoogle Scholar
- Cataldo F, Marino V, Ventura A, Bottaro G, Corazza GR: Prevalence and clinical features of selective immunoglobulin A deficiency in coeliac disease: an Italian multicentre study. Gut. 1998, 42 (3): 362-365.View ArticlePubMedPubMed CentralGoogle Scholar
- Korponay-Szabo IR, Dahlbom I, Laurila K, Koskinen S, Woolley N, Partanen J, Kovacs JB, Maki M, Hansson T: Elevation of IgG antibodies against tissue transglutaminase as a diagnostic tool for coeliac disease in selective IgA deficiency. Gut. 2003, 52 (11): 1567-1571. 10.1136/gut.52.11.1567.View ArticlePubMedPubMed CentralGoogle Scholar
- Ravelli A, Bolognini S, Gambarotti M, Villanacci V: Variability of histologic lesions in relation to biopsy site in gluten-sensitive enteropathy. Am J Gastroenterol. 2005, 100 (1): 177-185. 10.1111/j.1572-0241.2005.40669.x.View ArticlePubMedGoogle Scholar
- Hopper AD, Cross SS, Sanders DS: Patchy villous atrophy in adult patients with suspected gluten-sensitive enteropathy: is a multiple duodenal biopsy strategy appropriate?. Endoscopy. 2008, 40 (3): 219-224. 10.1055/s-2007-995361.View ArticlePubMedGoogle Scholar
- Green PH: Celiac disease: how many biopsies for diagnosis?. Gastrointest Endosc. 2008, 67 (7): 1088-1090. 10.1016/j.gie.2007.12.035.View ArticlePubMedGoogle Scholar
- Rostami K, Villanacci V: Microscopic enteritis: novel prospect in coeliac disease clinical and immuno-histogenesis. Dig Liver Dis. 2009, 41 (4): 245-252. 10.1016/j.dld.2008.06.008.View ArticlePubMedGoogle Scholar
- Paparo F, Petrone E, Tosco A, Maglio M, Borrelli M, Salvati VM, Miele E, Greco L, Auricchio S, Troncone R: Clinical, HLA, and small bowel immunohistochemical features of children with positive serum antiendomysium antibodies and architecturally normal small intestinal mucosa. Am J Gastroenterol. 2005, 100 (10): 2294-2298. 10.1111/j.1572-0241.2005.41134.x.View ArticlePubMedGoogle Scholar
- Tursi A, Brandimarte G: The symptomatic and histologic response to a gluten-free diet in patients with borderline enteropathy. J Clin Gastroenterol. 2003, 36 (1): 13-17. 10.1097/00004836-200301000-00006.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/9/57/prepub
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