Predictors of esophageal varices in patients with HBV-related cirrhosis: a retrospective study
© Hong et al; licensee BioMed Central Ltd. 2009
Received: 12 June 2008
Accepted: 05 February 2009
Published: 05 February 2009
All patients with liver cirrhosis are recommended to undergo an evaluation of esophageal varices (EV) to assess their risk of bleeding. Predicting the presence of EV through non-invasive means may reduce a large number of unnecessary endoscopies. This study was designed to develop a predictive model for varices in patients with Hepatitis B virus-related cirrhosis.
The retrospective analysis was performed in 146 patients with Hepatitis B virus-related cirrhosis. The data were assessed by univariate analysis and a multivariate logistic regression analysis. In addition, the receiver operating characteristic curves were also applied to calculate and compare the accuracy of the model and other single parameters for the diagnosis of esophageal varices.
We found the prevalence of EV in patients with Hepatitis B virus-related cirrhosis to be 74.7%. In addition, platelet count, spleen width, portal vein diameter and platelet count/spleen width ratio were significantly associated with the presence of esophageal varices on univariate analysis. A multivariate analysis revealed that only the spleen width and portal vein diameter were independent risk factors. The area under the receiver operating characteristic curve of regression function (RF) model, which was composed of the spleen width and portal vein diameter, was higher than that of the platelet count. With a cut-off value of 0.3631, the RF model had an excellent sensitivity of 87.2% and an acceptable specificity of 59.5% with an overall accuracy of 80.1%.
Our data suggest that portal vein diameter and spleen width rather than platelet count may predict the presence of varices in patients with Hepatitis B virus-related cirrhosis, and that the RF model may help physicians to identify patients who would most likely benefit from screenings for EV.
Most cirrhotic patients develop esophageal varices, with a lifetime incidence as high as 90% . Approximately one third of cirrhotic patients with esophageal varices (EV) develop an episode of esophageal hemorrhage, and subsequently have high morbidity and mortality . Therefore, early detection and prevention of EV in cirrhotic patients are crucial to minimizing complications. An endoscopy examination is currently considered to be the gold standard. However, screening all patients with endoscopy to guide therapy may significantly increase the cost.
Non-invasive indicators of varices are desired to reduce the need for screening endoscopy in all patients with cirrhosis. Studies shows that platelet count, splenomegaly, platelet count/spleen diameter ratio, advanced Child-Pugh class, serum albumin, and high portal vein diameter measured by ultrasonography may be useful non-invasive predictors of EV for patients with cirrhosis [2–4]. Such predictive factors may be expected to vary in different populations because of differences in the etiologies of liver cirrhosis and severity of liver disease. Data on this aspect in Chinese patients with liver cirrhosis, who usually have a higher proportion with hepatitis B viral etiology, remain largely unexplored. Here in this retrospective study, we evaluated the utility of various clinical, biochemical and ultrasonographic parameters in developing a model for predicting the presence of EV in Chinese patients with HBV-related cirrhosis.
This study included a total of 146 patients with HBV (Hepatitis B virus)-related cirrhosis that attended at our hospital between July 2005 and July 2007. Exclusion criteria included: active bleeding; current alcohol intake (patients with alcohol-related liver cirrhosis were excluded even if abstinent for at least 6 months prior to endoscopy); previous endoscopic sclerosis or band ligation of EV; previous surgery for portal hypertension or transjugular intrahepatic porto-systemic stent shunt placement. None of the patients were treated with β-blocker or diuretics. Hepatocellular carcinoma, spontaneous bacterial peritonitis, or portal vein thrombosis were also excluded for the study.
The following information was collected for each patient: age, gender, biochemical parameters including aspartate aminotransferase (AST), alanine aminotransferase (ALT), ALT/AST ratio, total bilirubin, conjugated bilirubin, total protein, serum albumin, prothrombin time, prothrombin activity (%), alkaline phosphatase, γ-glutamyl transpeptidase (GT), platelet count, presence and degree of ascites and encephalopathy assessed according to Child-Pugh criteria . Diuretics therapy was not commenced before endoscopy and ultrasonography was performed. The presence and size of EV were determined and recorded for each patient. The size of varices was subdivided into two classes–small and large according to the criteria proposed at the Baveno I Consensus Conference . Small EV were defined as varices that flatten with insufflation or minimally protrude into the esophageal lumen, while large EV were defined as varices that protrude into the esophageal lumen and touch each other (presence of confluence), or that fill at least 50% of the esophageal lumen. This study protocol was approved by the Ethic Committee of the First Affiliated Hospital of Wenzhou Medical College.
All patients underwent ultrasonographic examination of the upper abdomen including measurement of spleen width and portal vein diameter. The platelet count/spleen width ratio in all patients was then calculated. Ultrasonographic measurement of spleen width on a longitudinal section with the patient in the right lateral decubitus position was technically feasible in all patients as described by Lamb et al . The intra- and inter-observer coefficients of variation for measuring the spleen width were evaluated in 50 patients and were 1.2% and 2.1%, respectively.
All data were analyzed by STATA 10.0 software. Continuous values were expressed by mean ± SD or median and interquartile range and compared using the Student's t-test or the Mann-Whitney non-parametric test. Categorical values were described by count and proportions and compared by the χ2 test. Univariate analyses for determining the association of various clinical, laboratories and ultrasonographic variables with the presence or absence of EV were performed, and P-values below 0.05 were considered significant. All variables that were found to be different between patients with and without EV on univariate analysis were included as candidate variables in a forward-conditional step-wise logistic regression analysis to identify independent predictors for the presence of such varices. For this analysis, the conditional probabilities for stepwise entry and removal of a factor were 0.05 and 0.10, respectively. Based on the results of multiple logistic regression analysis, a logistic regression equation was developed to predict EV. The receiver operating characteristic curves (ROC curves) were applied to calculate and compare various predictors for the diagnosis of EV. The validity of the model was measured by means of the area under receiver operating characteristic curve (AUROC). A model with an AUROC above 0.7 was considered useful, while an AUROC between 0.8 and 0.9 indicated excellent diagnostic accuracy . The optimum cut-off value was chosen as the value corresponding with the highest accuracy (minimal false sensitivity and false positive results) for single variables, and various cut-off values were investigated for the model to determine the optimal cut-off values for predicting or excluding EV. The sensitivity (Se), specificity (Sp), negative predictive value (NPV), positive predictive value (PPV), and positive likelihood ratio (+LR), negative likelihood ratio (-LR) and diagnostic accuracy (DA) were calculated for various corresponding cut-off values.
Demographic and Clinical Characteristics of 146 patients
53.1 ± 12.2
Child-Pugh A/B/C (%)
Total Bilirubin (μmol/L)
45.5 ± 55.7
80.5 ± 70.8
61.8 ± 66.9
31.8 ± 6.3
Prothrombin time (s)
18.4 ± 4.5
61.5 ± 37.9
Portal vein diameter (mm)
12.6 ± 1.9
Spleen width (mm)
50.6 ± 10.1
Variables associated with the presence of EV by univariate analysis
Univariate analysis of predictive factors of EV in 146 patients
Absence of varices N = 37
Presence of varices N = 109
54.8 ± 13.6
52.5 ± 11.7
Total Bilirubin (μmol/L)
41.4 ± 40.7
46.9 ± 60.0
18.2 ± 21.0
20.6 ± 34.9
Total protein (g/L)
64.1 ± 5.3
63.2 ± 7.2
31.3 ± 6.2
31.9 ± 6.3
75.6 ± 95.8
57.0 ± 51.8
91.5 ± 99.1
76.7 ± 58.3
0.79 ± 0. 33
0. 77 ± 0. 27
Alkaline phosphatase (U/L)
109.4 ± 41.0
108.9 ± 70.9
γ- GT (U/L)
115.6 ± 139.1
82.3 ± 94.6
18.7 ± 5.5
18.3 ± 4.1
Prothrombin activity (%)
60.4 ± 17.9
60.7 ± 15.2
79.6 ± 51.1
55.3 ± 30.1
1.89 ± 1.39
1.15 ± 0.82
Spleen width (mm)
44.8 ± 7.6
52.5 ± 10.1
Portal vein diameter (mm)
11.6 ± 1.3
12.9 ± 1.9
Factors independently associated with EV
Logistic regression model to predict the presence of EV in 146 patients
Odds Ratio (95% Confidence interval)
Spleen width (mm)
Portal vein diameter (mm)
Diagnostic values of RF model, portal vein diameter, spleen width, platelet count and platelet count/spleen width ratio
Performance of the portal vein diameter, spleen width and the platelet count/spleen width ratio for predicting the presence of EV.
Portal vein diameter (mm)
Spleen width (mm)
Platelet count/Spleen width
Diagnostic values of RF model for predicting EV at various cut-off points.
Thrombocytopenia in patients with cirrhosis has historically been attributed to hypersplenism due to portal hypertension. Several studies suggest that platelet count may predict the presence of EV in patients with cirrhosis [2, 8, 9]. However, the discriminating threshold for the presence of varices varies widely, ranging between 68,000 and 160,000/mm3 . The sensitivities for thrombocytopenia fluctuate from 62% to 100%, and the specificities range from 18% to 77% . Our data suggest that the multivariate analysis failed to show any significant difference between thrombocytopenia and the risk of EV. In addition, platelet count might not be an ideal predictor of the presence of EV in HBV-related cirrhosis. A possible explanation is that other factors, such as suppressive effects of viruses on bone marrow and antibody-mediated destruction of platelets, may play a more important role in HBV-related cirrhosis than that in alcohol cirrhosis, in addition to decreased thrombopoeitin and interleukin-11 .
The prevalence of EV in this study is similar to previous findings . In addition, the prevalence of all varices in patients with advanced Child-Pugh class was higher than that in patients with Child-Pugh class A, which is also consistent with previous observations that patients with advanced liver disease are more likely to have varices . However, this difference did not reach statistical significance, which could be due to the small sample size of our study. Data on the relationship between Child-Pugh score and the risk of EV is somewhat conflicting. One study showed that Child-Pugh score was an acceptable predictor of the presence of EV , whereas a recent well-designed study suggested that it was not a useful predictor . Our data do not support any correlation between Child-Pugh score with varices, which may partially be due to a rather homogeneous population in the present study, in which 50% of patients were Child-Pugh class B. As suggested by Rajvanshi et al, the prevalence of EV increased with a higher Child-Pugh class, but Child-Pugh score was not a predictor of EV .
EV is the direct consequence of spontaneous formation of collateral vessels between portal vein and esophageal veins via left gastric or short gastric veins. Therefore, the presence or absence of EV may reflect the severity of portal hypertension. In a logistic regression study of 143 patients , ultrasonographic portal vein diameter greater than 13 mm was one of the independent risk factors for the presence of EV. However, another study  failed to confirm the predicting role of portal vein diameter when using a cut-off value of 13 mm in prevalently HCV-related cirrhosis. The results of the present study indicate that the portal vein diameter with an AUROC of 0.739 could be a valuable predictor of EV in patients with HBV-related cirrhosis. The fact that different studies conclude different best cut-off values of the portal vein diameter may be explained at least in part by that the physique of Asian populations is smaller than that of European populations. It has been reported that the normal mean portal vein diameter in Chinese populations is 9.5 ± 1.3 mm , while it is 11.0 ± 0.3 mm in French populations .
Splenomegaly is recognized as one of the diagnostic signs of cirrhosis and portal hypertension. Several studies show that splenomegaly may be a good predictor of EV [2, 9, 20, 21]. Lamb et al found that there was a good correlation between in vivo ultrasound assessment of splenic width and true splenic volume . Our data showed that spleen width measured by ultrasonography was an independent predictor for the presence of varices with an AUROC of 0.736. Contrary to what was suggested in previous reports, no correlation between splenomegaly and EV was found in other studies [17, 22, 23]. These differences may be due to the variations among studies regarding the etiology and the stage of liver cirrhosis studied. Moreover, splenomegaly is found more frequently in posthepatitic cirrhosis than in alcoholic cirrhosis . With the best cut-off value of 1.0513 (109/L)/mm, the platelet count/spleen width ratio yielded a low diagnostic accuracy of 60.3%, which suggests that it is not an ideal predictor for EV.
With a cut-off value of 0.3631, the RF model had an excellent sensitivity of 87.2% and an acceptable specificity of 59.5% with an overall accuracy of 80.1%, which was higher than that of spleen width and portal vein diameter. And with a cut-off value of 0.1705, 82.2% of the patients were correctly classified. However, our data showed that the RF model had a moderate predictive power with an AUROC of 0.777. Moreover, the other parameters listed in Table 5 are also modest: the positive likelihood ratios of the different cut-off values are rather low, and the negative likelihood ratios are too high except for the cut-off -0.3052. However, the specificity for the cut-off -0.3052 is fairly low, which means that some of these patients with splenomegaly and dilated portal vein may not have EV. One of the possible explanations for this result could be the development of spontaneous intra-abdominal shunts that decrease the blood flow of varices while maintaining congestive splenomegaly and dilated portal vein.
Our study has several limitations. First, the data were collected retrospectively, so patients at a relatively more advanced stage of the disease were more prone to be enrolled in our study, while outpatients were more likely to be excluded for insufficient data, which may produce a population bias. Second, prediction models may vary with the nature of the patient population from which these are derived. The population of our study is largely composed of patients with decompensated cirrhosis, and only partially reflects the population in which an index to predict the presence of varices would be mostly applied in clinical practice, i.e. patients enrolled at the time of the diagnosis of cirrhosis. This might have artificially increased the performance of the prognostic index. The predictability of variables in the RF model might not apply to a patient or cohort with less advanced cirrhosis. Prospective validation in an independent patient population is mandatory. Whereas the authors have to state clearly that until this is done, the model should not be used as a substitute for endoscopy because the parameters of the model are all modest. Third, the performance characteristics of the index is globally modest – the AUROC is inferior to that of other indexes such as the platelet count/spleen diameter ratio [4, 25, 26], CT esophagography , all of which are recently considered as excellent predictors for EV. It will be interesting and necessary to analyze these predictors in comparison in future studies.
Although the present study has the above-mentioned limitations, it is the first study, to the best of our knowledge, to investigate predictors of EV in HBV-related cirrhosis in homogeneous population. In addition, the platelet count/spleen width ratio and the spleen width on ultrasonography were first introduced as potential predictors of EV. The intra- and inter-observed variability for the spleen width measurement is good, so it is reproducible. The RF model with a certain cut-off value has a much higher sensitivity and NPV for ruling out the presence of EV and higher diagnostic accuracy than that of single predictors such as the platelet count/spleen width ratio, the spleen width, the portal vein diameter.
Portal vein diameter and spleen width but not platelet count may predict the presence of varices in hepatitis B virus-related cirrhosis. The RF model may also help clinicians to identify patients who would most likely benefit from referrals for screening for esophageal varices. However, these findings, including the validity of the model, need to be verified with prospectively collected data. Endoscopic screening for EV may be recommended in HBV-related cirrhotic patients with splenomegaly and dilated portal vein.
This work was partially supported by a 5010 grant of Wenzhou Medical College.
- Chang YW: Indication of treatment for esophageal varices: who and when?. Digestive Endoscopy. 2006, 18 (1): 10-15. 10.1111/j.1443-1661.2006.00579.x.View ArticleGoogle Scholar
- Chalasani N, Imperiale TF, Ismail A, Sood G, Carey M, Wilcox CM, Madichetty H, Kwo PY, Boyer TD: Predictors of large esophageal varices in patients with cirrhosis. Am J Gastroenterol. 1999, 94 (11): 3285-3291. 10.1111/j.1572-0241.1999.1539_a.x.View ArticlePubMedGoogle Scholar
- Zardi EM, Uwechie V, Gentilucci UV, Dobrina A, Petitti T, Laghi V, Picardi A, Afeltra A: Portal diameter in the diagnosis of esophageal varices in 266 cirrhotic patients: which role?. Ultrasound Med Biol. 2007, 33 (4): 506-511. 10.1016/j.ultrasmedbio.2006.10.002.View ArticlePubMedGoogle Scholar
- Giannini EG, Zaman A, Kreil A, Floreani A, Dulbecco P, Testa E, Sohaey R, Verhey P, Peck-Radosavljevic M, Mansi C, et al: Platelet count/spleen diameter ratio for the noninvasive diagnosis of esophageal varices: results of a multicenter, prospective, validation study. Am J Gastroenterol. 2006, 101 (11): 2511-2519. 10.1111/j.1572-0241.2006.00874.x.View ArticlePubMedGoogle Scholar
- Pugh RN, Murray-Lyon IM, Dawson JL, Pietroni MC, Williams R: Transection of the oesophagus for bleeding oesophageal varices. The British journal of surgery. 1973, 60 (8): 646-649. 10.1002/bjs.1800600817.View ArticlePubMedGoogle Scholar
- de Franchis R, Pascal JP, Ancona E, Burroughs AK, Henderson M, Fleig W, Groszmann R, Bosch J, Sauerbruch T, Soederlund C, et al: Definitions, methodology and therapeutic strategies in portal hypertension. A Consensus Development Workshop, Baveno, Lake Maggiore, Italy, April 5 and 6, 1990. Journal of hepatology. 1992, 15 (1–2): 256-261. 10.1016/0168-8278(92)90044-P.View ArticlePubMedGoogle Scholar
- Lamb PM, Lund A, Kanagasabay RR, Martin A, Webb JA, Reznek RH: Spleen size: how well do linear ultrasound measurements correlate with three-dimensional CT volume assessments?. The British journal of radiology. 2002, 75 (895): 573-577.View ArticlePubMedGoogle Scholar
- Zaman A, Becker T, Lapidus J, Benner K: Risk factors for the presence of varices in cirrhotic patients without a history of variceal hemorrhage. Arch Intern Med. 2001, 161 (21): 2564-2570. 10.1001/archinte.161.21.2564.View ArticlePubMedGoogle Scholar
- Thomopoulos KC, Labropoulou-Karatza C, Mimidis KP, Katsakoulis EC, Iconomou G, Nikolopoulou VN: Non-invasive predictors of the presence of large oesophageal varices in patients with cirrhosis. Dig Liver Dis. 2003, 35 (7): 473-478. 10.1016/S1590-8658(03)00219-6.View ArticlePubMedGoogle Scholar
- de Franchis R: Noninvasive diagnosis of esophageal varices: is it feasible?. Am J Gastroenterol. 2006, 101 (11): 2520-2522. 10.1111/j.1572-0241.2006.00880.x.View ArticlePubMedGoogle Scholar
- D'Amico G, Morabito A: Noninvasive markers of esophageal varices: another round, not the last. Hepatology. 2004, 39 (1): 30-34. 10.1002/hep.20018.View ArticlePubMedGoogle Scholar
- Charuworn P, Cheung R: Screening for esophageal varices in cirrhotic patients: endoscope, platelet count, or both?. Gastrointest Endosc. 2006, 64 (6): 865-867. 10.1016/j.gie.2006.08.033.View ArticlePubMedGoogle Scholar
- Jensen DM: Endoscopic screening for varices in cirrhosis: findings, implications, and outcomes. Gastroenterology. 2002, 122 (6): 1620-1630. 10.1053/gast.2002.33419.View ArticlePubMedGoogle Scholar
- Thabut D, Trabut JB, Massard J, Rudler M, Muntenau M, Messous D, Poynard T: Non-invasive diagnosis of large oesophageal varices with FibroTest in patients with cirrhosis: a preliminary retrospective study. Liver Int. 2006, 26 (3): 271-278. 10.1111/j.1478-3231.2005.01227.x.View ArticlePubMedGoogle Scholar
- Qamar AA, Grace ND, Groszmann RJ, Garcia-Tsao G, Bosch J, Burroughs AK, Maurer R, Planas R, Escorsell A, Garcia-Pagan JC, et al: Platelet count is not a predictor of the presence or development of gastroesophageal varices in cirrhosis. Hepatology. 2008, 47 (1): 153-159. 10.1002/hep.21941.View ArticlePubMedGoogle Scholar
- Rajvanshi P, Kowdley KV: Prediction of varices in patients with cirrhosis: a high-stakes numbers game?. Journal of clinical gastroenterology. 2002, 34 (1): 4-5. 10.1097/00004836-200201000-00003.View ArticlePubMedGoogle Scholar
- Schepis F, Camma C, Niceforo D, Magnano A, Pallio S, Cinquegrani M, D'Amico G, Pasta L, Craxi A, Saitta A, et al: Which patients with cirrhosis should undergo endoscopic screening for esophageal varices detection?. Hepatology. 2001, 33 (2): 333-338. 10.1053/jhep.2001.21410.View ArticlePubMedGoogle Scholar
- He YK, Zhou H, Sun KY, Huang LX, Zhao XG, Li Y, Luo XS, Christoph H, Li YS: A Study on Establishing a Normal Standard of Liver and Spleen for Ultrasonographic Measurement. Practical Preventive Medicine. 2004, 11 (3): 420-422.Google Scholar
- Tasu JP, Rocher L, G PE, Kuoch V, Kulh E, Miquel A, Buffet C, BlEry M: Hepatic venous pressure gradients measured by duplex ultrasound. Clinical radiology. 2002, 57 (8): 746-752. 10.1053/crad.2002.0951.View ArticlePubMedGoogle Scholar
- Madhotra R, Mulcahy HE, Willner I, Reuben A: Prediction of esophageal varices in patients with cirrhosis. Journal of clinical gastroenterology. 2002, 34 (1): 81-85. 10.1097/00004836-200201000-00016.View ArticlePubMedGoogle Scholar
- Sharma SK, Aggarwal R: Prediction of large esophageal varices in patients with cirrhosis of the liver using clinical, laboratory and imaging parameters. Journal of gastroenterology and hepatology. 2007, 22 (11): 1909-1915. 10.1111/j.1440-1746.2006.04501.x.View ArticlePubMedGoogle Scholar
- Pilette C, Oberti F, Aube C, Rousselet MC, Bedossa P, Gallois Y, Rifflet H, Cales P: Non-invasive diagnosis of esophageal varices in chronic liver diseases. Journal of hepatology. 1999, 31 (5): 867-873. 10.1016/S0168-8278(99)80288-8.View ArticlePubMedGoogle Scholar
- Burton JR, Liangpunsakul S, Lapidus J, Giannini E, Chalasani N, Zaman A: Validation of a multivariate model predicting presence and size of varices. Journal of clinical gastroenterology. 2007, 41 (6): 609-615. 10.1097/01.mcg.0000225669.84099.04.View ArticlePubMedGoogle Scholar
- Bolondi L, Zironi G, Gaiani S, Li Bassi S, Benzi G, Barbara L: Caliber of splenic and hepatic arteries and spleen size in cirrhosis of different etiology. Liver. 1991, 11 (4): 198-205.View ArticlePubMedGoogle Scholar
- Giannini E, Botta F, Borro P, Risso D, Romagnoli P, Fasoli A, Mele MR, Testa E, Mansi C, Savarino V, et al: Platelet count/spleen diameter ratio: proposal and validation of a non-invasive parameter to predict the presence of oesophageal varices in patients with liver cirrhosis. Gut. 2003, 52 (8): 1200-1205. 10.1136/gut.52.8.1200.View ArticlePubMedPubMed CentralGoogle Scholar
- Giannini EG, Botta F, Borro P, Dulbecco P, Testa E, Mansi C, Savarino V, Testa R: Application of the platelet count/spleen diameter ratio to rule out the presence of oesophageal varices in patients with cirrhosis: a validation study based on follow-up. Dig Liver Dis. 2005, 37 (10): 779-785. 10.1016/j.dld.2005.05.007.View ArticlePubMedGoogle Scholar
- Kim YJ, Raman SS, Yu NC, To'o KJ, Jutabha R, Lu DS: Esophageal varices in cirrhotic patients: evaluation with liver CT. AJR Am J Roentgenol. 2007, 188 (1): 139-144. 10.2214/AJR.05.1737.View ArticlePubMedGoogle Scholar
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