Colectomy rate in steroid-refractory colitis initially responsive to cyclosporin: a long-term retrospective cohort study
© Actis et al; licensee BioMed Central Ltd. 2007
Received: 09 August 2006
Accepted: 27 March 2007
Published: 27 March 2007
There is consistent evidence that 50% of patients with acute, steroid-resistant flare of ulcerative colitis (UC) may achieve remission and avoid colectomy if treated with cyclosporin (CsA). However, follow-up of the responders has shown that most of them relapse and need surgery shortly after the response. We compared the records of our CsA-treated patients with those of other groups in order to help clarify this matter.
All patients admitted consecutively to our Unit with an attack of UC and treated with CsA between January 1991 and December 1999 were studied. Patients were begun on continuously-infused CsA at 2 mg/kg/day (1991–1996), or on NEORAL at an initial dose of 5 mg/kg/day (1996–1999). The maintenance treatment included oral CsA for 3–6 months with or without azathioprine (AZA). CsA failure was defined as a relapse requiring steroids with or without progression to colectomy; the cumulative probability of relapse/colectomy was assessed by Fisher's exact tests and Kaplan-Meier analysis.
Among the patients, 39/61 (63%) initially responded. These 39 included a fatality and 4 drop-outs (unrelated to the side-effects of CsA), leaving 34 patients for the study. Of these, 61% and 35% were colectomy-free at 1 and 7 years, respectively; the corresponding figures were 80 and 60% respectively in the subset treated with AZA, but 47% and 15% in the AZA-untreated subgroup (p= 0.0007 at 7 years). Among the 34 patients, 44% were relapse-free at 1 year, but all had relapsed at 7 years (p = 0.0635). The overall resort to colectomy was 72%, while 19% of the patients remained colectomy-free.
Sixty percent of a cohort of patients with steroid-refractory colitis responded to CsA and 60% of these responders retained the colon after 1 year. These figures fell to 35% at 7 years but improved to 60% on AZA. The overall need for colectomy remains high in these patients and toxicity must be monitored.
On the basis of the positive experience achieved in controlling organ rejection in the previous decade, the use of CsA to control acute steroid-refractory flares of UC was begun in the 1990s , when steroid unresponsiveness constituted a mandatory indication for colectomy in ~40% of the affected patients . A controlled, albeit small, study soon showed that CsA can eliminate the need for immediate colectomy in this context , but the long-term efficacy of the drug in the treatment of UC has not been clearly established and remains a controversial issue. Fifteen years after the introduction of CsA to treat refractory colitis, several authors reviewed their follow-up data in an attempt to clarify the uncertainties concerning its long-term effects [4–8]. This study represents a comparative analysis between our results in treating acute steroid-refractory flares with CsA and the results obtained by others.
Patients' baseline characteristics
Mean Age (yrs)
34 ± 12
Disease Duration (months)
47 ± 7
% disease beyond splenic flexure
% Previous ASA use
% Previous AZA use
% Previous steroid use
The average daily stool passage in the Neoral subset was 7 ± 3, the first hour sedimentation rate was 53 ± 25, and the hemoglobin concentration was 10 ± 2, yielding a CAI score of 11 ± 3. As previously shown , the frequency of left-sided disease was the same as that in the IV treated subset.
Of the initial responders, those responding to Neoral were immediately placed on maintenance regimes; those responding to the induction phase with IV CsA were treated similarly after a 6-month phase of 6–8 mg/kg of conventional (oral) CsA. The maintenance regimes consisted of therapeutic doses of mesalamine or mesalamine derivatives, except for 4 cases, of whom one developed a reduced platelet count when receiving mesalamine. A subset of 15 responders also received AZA at an initial dose of 2 mg/kg, either concurrently with CsA (11 patients) or at the end of the course (4 cases), as detailed in the Results section.
Responses and toxicity were monitored as previously described [11, 12]. By the initial response, we intended to avoid immediate colectomy. Cyclosporin failure was defined as disease relapse requiring steroids, with or without progression to colectomy.
Data were first analyzed by descriptive statistics. Differences between groups were tested by Student's t-test, chi-square and Fisher's exact test, as appropriate. The cumulative probability and recurrence of relapse/colectomy was calculated using the actuarial life table analyses of Kaplan-Meier, using the log-rank test to compare the curves. Significance was assigned if P < 0.05. All the statistical analyses were performed using StatsDirect statistical software Version 2.5.7.
Factors of immediate response to CsA or immediate failure*
Patients' age at onset (yrs)
31 ± 9
28 ± 11
Age at inclusion (yrs)
36 ± 10
34 ± 12
Disease Duration (months)
57.9 ± 13
51 ± 6
% universal disease
% transfusion dependent
% TPN dependent
White Blood Count (per μλ)
13.9 ± 7.9 × 103
13.0 ± 4.8 × 103
CsA levels (ng/ml whole blood)
251 ± 94
210 ± 37
Overview of the results achieved in the current cohort study.
All Patients (n = 34)
With AZA (n = 15)
Without AZA (n = 19)
No colectomy > 1 yr
No colectomy > 3 yrs
No colectomy > 7 yrs
No relapse > 1 yr
No relapse > 3 yrs
No relapse >7 yrs
Major side effects observed during the intravenous and oral phases of CsA administration
>33% ↑ creatinine
2 × creatinine
↑ liver function tests
In this retrospective study, we analyzed the immediate response and the outcome of rescue therapy with CsA in a cohort of patients whom we treated between 1991 and 1999. The patients included in this study had failed to respond to a course of full-dose parenteral steroids, and were therefore diagnosed with refractory UC, with the attendant threat of colon surgery. The findings are consistent with those from the sources quoted in the Background and indicate that: (i) the administration of IV or oral CsA can eliminate the need for immediate colectomy in 60–80% of patients with steroid-refractory UC; (ii) most of the responders will relapse with or without progression to colectomy in the following 3–7 years; (iii) the cumulative risk for colectomy in initial responders can be significantly reduced by the use of AZA as a long-acting maintenance drug. Only the report of Moskowitz is not clearly consistent with the last point. These authors did not formally conduct a comparative Kaplan-Meier analysis of the risk for colectomy in the AZA-treated versus the AZA-untreated subjects in their cohort. Rather, they claimed that de-novo treated patients were at lower risk for colectomy than those already treated with AZA at the time of the initial CsA course. The data in our paper are in fact similar to Moskowitz's findings (see the Results) and we can probably concur with them in suggesting that the AZA-pretreated subjects exhibit the poorest response to CsA in this context. However, the proportion of such patients in our study was only 11% (31% in Moskowitz's paper), which precluded stratification by Kaplan-Meier analysis. This low percentage of "bad responder" patients may be reflected in our achievement of a higher proportion of colectomy-free patients at the 7th year of follow-up (35% versus 12%).
It is intriguing that while specialized UC centers like our own tend to focus on the negative side of their results, primary care centers highlight the availability of CsA for their patients and emphasize its effectiveness . The data may indeed have a positive aspect: (i) if tempted to consider the action of CsA as limited or short-lived, one should take into account that all the patients enrolled in the CsA studies were by definition heading for colectomy; (ii) the often-emphasized toxicity of CsA can be controlled when the drug is administered against the background of an organ-transplant program, and can be further decreased by low-dosage regimens. The use of low-dose CsA to treat refractory colitis was originally proposed by us  in an uncontrolled study and later validated independently by other authors . Finally, a recent controlled study has shown that low-dose CsA is as effective as high-dose . Low-dose treatment might obviously impact on the toxicity figures. The rates observed in this work, although not negligible, are lower than those recorded in other high-dose trials, where for example infection and renal damage peaked to 20% . The desirability of low-dose schedules has been similarly emphasized in recently published therapeutic guidelines , which also suggest that better patient selection and the use of purine analogues can improve the pharmacological profile of CsA in its indication for UC.
The novel biological drugs that have been introduced recently to treat UC have addressed moderately severe forms of colitis [18, 19] and it is felt that their indication for treating the most severe forms of the disease is premature . Also, two controlled studies published independently have come to opposite conclusions [20, 21]; it may also be noted that in the study with positive findings, by Järnerot, the patients who fared worse were those with steroid-refractory disease. Doubts about the effectiveness of anti-TNF strategies in patients hospitalized with steroid-refractory disease have also been expressed in a recently published analysis .
For patients with severe steroid-refractory UC, a strategy based on the use of CsA as an inductive therapy is effective; the progressive occurrence of relapses (eventually followed by colectomy) that follows the initial response to CsA can be significantly reduced (but not abolished) by the long-term use of a thiopurine drug (AZA); patients already on AZA will benefit least from this strategy.
= Aminosalicylic acid
= Rachmilewitz score
= Tumor Necrosis Factor
= Total Parenteral Nutrition
= Ulcerative Colitis
This work was partly supported by AIRC regional funds 2005, and was selected for presentation and publication in abstract form at UEGW 2006. Marco Pinna-Pintor was the reference surgeon; Marco Astegiano directed the out-patient clinic.
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