Follow-up of pediatric celiac disease: value of antibodies in predicting mucosal healing, a prospective cohort study
- Edith Vécsei†1,
- Stephanie Steinwendner†1,
- Hubert Kogler1,
- Albina Innerhofer1,
- Karin Hammer1,
- Oskar A Haas1,
- Gabriele Amann2,
- Andreas Chott3,
- Harald Vogelsang4,
- Regine Schoenlechner5,
- Wolfgang Huf6 and
- Andreas Vécsei1Email author
© Vécsei et al.; licensee BioMed Central Ltd. 2014
Received: 30 January 2014
Accepted: 3 February 2014
Published: 13 February 2014
In diagnosing celiac disease (CD), serological tests are highly valuable. However, their role in following up children with CD after prescription of a gluten-free diet is unclear. This study aimed to compare the performance of antibody tests in predicting small-intestinal mucosal status in diagnosis vs. follow-up of pediatric CD.
We conducted a prospective cohort study at a tertiary-care center. 148 children underwent esophohagogastroduodenoscopy with biopsies either for symptoms ± positive CD antibodies (group A; n = 95) or following up CD diagnosed ≥ 1 year before study enrollment (group B; n = 53). Using biopsy (Marsh ≥ 2) as the criterion standard, areas under ROC curves (AUCs) and likelihood-ratios were calculated to estimate the performance of antibody tests against tissue transglutaminase (TG2), deamidated gliadin peptide (DGP) and endomysium (EMA).
AUCs were higher when tests were used for CD diagnosis vs. follow-up: 1 vs. 0.86 (P = 0.100) for TG2-IgA, 0.85 vs. 0.74 (P = 0.421) for TG2-IgG, 0.97 vs. 0.61 (P = 0.004) for DPG-IgA, and 0.99 vs. 0.88 (P = 0.053) for DPG-IgG, respectively. Empirical power was 85% for the DPG-IgA comparison, and on average 33% (range 13–43) for the non-significant comparisons. Among group B children, 88.7% showed mucosal healing (median 2.2 years after primary diagnosis). Only the negative likelihood-ratio of EMA was low enough (0.097) to effectively rule out persistent mucosal injury. However, out of 12 EMA-positive children with mucosal healing, 9 subsequently turned EMA-negative.
Among the CD antibodies examined, negative EMA most reliably predict mucosal healing. In general, however, antibody tests, especially DPG-IgA, are of limited value in predicting the mucosal status in the early years post-diagnosis but may be sufficient after a longer period of time.
KeywordsPediatrics Celiac disease Follow-up Endomysial antibodies Sensitivity Specificity
Celiac disease (CD) is a multi-systemic autoimmune disease triggered by exposure to dietary gluten in genetically predisposed individuals. CD creates small-intestinal mucosal injury of different severity . An effective treatment allowing mucosal healing is the gluten-free diet (GFD). The goals of treatment are not only symptomatic improvement but also avoiding complications, which could arise even in patients having become asymptomatic on a GFD [2, 3]. Furthermore, achieving mucosal healing might be crucial because of an increased risk of lymphoproliferative malignancy among patients with persistent villous atrophy .
International CD guidelines propose regular follow-up of CD patients [5–7]. Among the follow-up modalities, re-biopsy may be undertaken to prove mucosal healing, which children achieve more often than adults . However, its invasiveness, discomfort and possible complications limit the use of re-biopsy in routine follow-up [5, 6]. Therefore, reliable non-invasive surrogate markers of mucosal healing are highly desirable. Whereas antibody tests are of irreplaceable value in diagnosing untreated CD , controversy exists over whether these tests can reliably indicate mucosal healing [8–11].
Concerning the correlation between follow-up histology and non-invasive biomarkers, children with CD are an understudied population. Specifically, there is a lack of prospective pediatric studies evaluating current biomarkers used in clinical practice for monitoring purposes.
The purpose of this study was to prospectively compare the performance of up-to-date antibody tests in predicting mucosal status in children with untreated CD vs. in children after prescription of a GFD.
Study design and subjects
Group A comprised 95 children on a gluten-containing diet, 32 of them became diagnosed with CD (group A1) and 63 were referred to EGD due to non-celiac dyspepsia (group A2). The predominant complaints in group A1 children were abdominal pain (31.3%), failure to thrive or short stature (18.8%), chronic diarrhea (6.3%), flatulence (6.3%), recurrent headache (6.3%) and constipation (3.1%). A first-degree relative with CD (18.8%), IgA-deficiency (3.1%), autoimmune thyroiditis (3.1%), and iron deficiency anemia (3.1%) were the remaining reasons for CD screening in group A1. Diagnosis of CD was based on positive IgA antibodies against endomysium (EMA) in IgA-competent children or IgG-antibodies against deamidated gliadin peptides (DGP-IgG) in children with IgA-deficiency along with biopsy results consistent with CD (Marsh ≥ 2) and positivity of HLA-DQ2 and/or HLA-DQ8. CD was ruled out by negative biopsy results.
Group B comprised 53 children with CD after prescription of a GFD ≥ 1 year before study enrollment (median 2.2, range 1 to 12.9). CD had been proven by positive EMA or IgA antibodies against tissue transglutaminase (TG2-IgA), biopsy evidence and positivity of HLA-DQ2 and/or HLA-DQ8. Group B children had received regular follow-up according to the recommendations then in force . Within the 18-month study period, a total of 79 children presented for routine CD follow-up. All of these children were invited to participate in the study independent of the presence of symptoms or their adherence to the GFD according to dietary interview. As such they were unselected and only chosen by their willingness to undergo follow-up endoscopy. In this context, 26 of 79 eligible children opted out of the study. The predominant complaints in group B were abdominal pain (15.1%), constipation (1.9%) and aphthous stomatitis (1.9%). Within group B, 79.2% of children were symptom-free.
Endoscopy, biopsies and histology
All EGDs were performed in anesthesiologist-controlled deep sedation with propofol. Four biopsies were taken from the second part and two from the bulb of the duodenum. Biopsies were staged by two experienced pathologists (GA and AC) who were blinded to subject identity and indication for biopsy. Intestinal histological findings were classified according to a modified Marsh classification  using ≥ 30 lymphocytes/100 epithelial cells as cut-off for pathological intraepithelial lymphocytosis . In cases of initial disagreement, a consensus diagnosis was reached using a multihead microscope. Mucosal healing in group A was defined as Marsh < 2.
Antibody tests for celiac disease used in the study
Manufacturer’s cut-off (U/ml)
Optimal ROC cut-off for diagnosing untreated CD (U/ml)
Optimal ROC cut-off for monitoring treated CD (U/ml)
Endomysial IgA antibodies assay
ORG 802/ORG 872 Anti-Endomysium Antibodies kit
Orgentec Diagnostika GmbH, Mainz, Germany
Anti-tissue transglutaminase assays
Eu-tTG umana IgA, Eurospital, Trieste, Italy
Quanta Lite h-tTG IgG ELISA, Inova Diagnostics
Antibodies against deamidated gliadin peptide assays
Quanta Lite Gliadin IgA II ELISA, Inova Diagnostics, San Diego, USA
Quanta Lite Gliadin IgG II ELISA, Inova Diagnostics, San Diego, USA
Data of continuous and categorical variables were reported using median and interquartile range (IQR) on the one hand and counts and percentages on the other hand. For comparisons on categorical data chi-square and Fisher exact test were used while Mann–Whitney U and Kruskal-Wallis tests were used to compare continuous data. Unless otherwise specified, Bonferroni correction was applied for multiple comparisons in post-hoc-tests. Kappa coefficients were used to examine agreement among pathologists in classifying histological findings. Performances of non-invasive tests were evaluated by ROC curve analysis. Areas under ROC curves (AUC), summary measures of overall diagnostic performance, were reported with their 95% confidence intervals (CIs); AUC > 0.9 was considered a high diagnostic test performance . To rank the performance of tests when used for diagnosing CD, AUCs of ROC curves derived from group A were compared . To rank the performance of tests when used for follow-up monitoring of CD, AUCs of ROC curves derived from group B cases were compared. Furthermore, we compared the performance of each single test when used for diagnosis vs. for follow-up monitoring of CD . We also used ROC curve analysis to determine the optimal cut-off point for each test and calculated further performance measures like sensitivity, specificity, positive and negative likelihood-ratios (LR + and LR-) with 95% CIs. Where there was a 2x2 table with an empty cell 0.5 was added to each cell. Tests with either a LR+ > 10 or LR- < 0.1 were considered informative and clinically useful . Statistical calculations were performed with SPSS, version 20.0 (SPSS, Chicago, Ill), and the free software R . For all statistical analyses, Bonferroni corrected two-tailed P-values < 0.05 were considered significant.
The Institutional Review Board at St. Anna Children’s Hospital approved this study. Since obtaining follow-up biopsies in children with CD deviates from our clinical practice, all group B children were covered by a clinical trial insurance policy valid throughout the 18-month study period.
Age of the 148 children ranged from 2 to 19 years (median 11.3). Children with newly diagnosed CD (group A1) were younger (median age in years [IQR, min to max]: 7.8 [6.2, 2.0 to 17.6]) than both dyspeptic children (group A2) (median age in years [IQR, min to max]: 12.6 [5.3, 3.3 to 18.0]) and group B children (median age in years [IQR, min to max]: 11.8 [4.8, 4.2 to 19.1]) (P < 0.001). In all groups, girls outnumbered boys (girls/boys in group A1: 23/9; in group A2: 48/15; in group B: 33/20) (P = 0.26).
Performance of antibody tests in group A
Performance of antibody tests in diagnosing untreated celiac disease and monitoring treated celiac disease
Primary diagnosis of untreated celiac disease (Group A, n = 97)
0.85 to 1.00
0.90 to 1.00
0.99 to 1.00
8.62 to 204.13
0.000 to 0.270
0.52 to 0.86
0.75 to 0.93
0.77 to 0.93
2.61 to 9.47
0.190 to 0.590
0.85 to 1.00
0.53 to 0.97
1.79 to 16.95
0.000 to 0.320
Antibodies against deamidated gliadin peptides
0.76 to 0.99
0.85 to 0.98
0.94 to 1.00
5.63 to 37.96
0.020 to 0.290
0.83 to 1.00
0.87 to 0.99
0.98 to 1.00
6.72 to 61.43
0.000 to 0.230
Monitoring treated celiac disease (Group B, n = 53)
0.36 to 1.00
0.74 to 0.95
0.69 to 1.00
2.85 to 14.95
0.032 to 1.147
0.22 to 0.96
0.64 to 0.89
0.49 to 0.99
1.42 to 6.90
0.135 to 1.326
0.50 to 1.00
0.59 to 0.86
0.80 to 0.98
2.12 to 5.99
0.070 to 1.403
Antibodies against deamidated gliadin peptides
0.22 to 0.96
0.51 to 0.79
0.38 to 0.84
0.98 to 3.91
0.160 to 1.596
0.36 to 1.00
0.62 to 0.88
0.76 to 0.99
1.90 to 6.68
0.036 to 1.311
Within group A1, all children had positive EMA, positive EMA being an inclusion criterion. Therefore, no performance evaluation for EMA using ROC curve analysis was done in group A.
Within group A2, positive EMA were found in 3 children (specificity 0.94; 95% CI 0.86 to 0.99) who all had Marsh 0 as the result of the small intestinal biopsy. Of these EMA-positive group A2 children, 2 tested positive for TG2-IgA as well. In 2 children, all antibody titres normalized on follow-up under gluten-containing diet within 6 months. One these two children still had Marsh 2 in a follow-up biopsy 28 months later. The third EMA-positive group A2 child was placed on a GFD by her parents for 2.5 years before presenting for follow-up visit. At this visit, she was seronegative. She is currently undergoing a gluten challenge from November 2012 onwards. At her last visit in October 2013 she was still seronegative.
Performance of antibody tests in group B
Comparing the performance of antibody tests in predicting mucosal status in group A vs. B, all tests performed less well in group B (Figures 2 and 3, Table 2). This performance loss was significant at an uncorrected level of alpha = 0.05 in case of DPG-IgA (P uncorr. = 0.004). Empirical power (calculated using 1000 bootstrap samples) was 85% for this comparison, and on average 33% (range 13–43) for the non-significant comparisons. Within group B, EMA performed best followed by DGP-IgG and TG2-IgA.
Among the LRs obtained in group B, only the negative LR of EMA was low enough (0.097) to effectively rule out persistent mucosal injury (Table 2).
However, within group B, positive EMA were detected in 18 children, 12 of whom exhibited mucosal healing. Of these children with EMA-positivity despite mucosal healing, all 12 had Marsh 0 as the result of the small intestinal biopsy; 9 (75%) became EMA-negative on further follow-up within the next 20 months. Before study enrollment, all of these 12 children initially had EMA ≥ 1:160 and Marsh ≥ 3A at diagnosis except one girl with EMA 1:80 and Marsh 2. On enrollment, all these children still had positive EMA ranging from 1:5 to 1:80 despite Marsh 0 in the study biopsies after a median of 1.5 years on a GFD. Comparing the duration of the GFD in group B children with mucosal healing, EMA-positive children had been on the GFD for a shorter period of time (median age in years [IQR, min to max]: 1.5 [0.8, 1.0 to 3.0]) than the EMA-negative children (median age in years [IQR, min to max]: 2.3 [5.5, 1.0 to 12.9]) (P = 0.008).
Overall, of the group B children exhibiting Marsh 0, 4 had positive TG2-IgA, 15 positive TG2-IgG, 12 positive EMA, 16 positive DPG-IgA, and 10 positive DPG-IgG. All 35 EMA-negative group B children with mucosal healing (34 children with Marsh 0 and one with Marsh 1) also had negative TG2-IgA.
In group A1, where all children had histology consistent with CD, severe mucosal injury (Marsh 3B or 3C) was found in 28 children (87.5%) while the remaining 4 children (12.5%) showed Marsh 3a. In group A2, histology showed gastritis in 39 children, esophagogastritis in 8, esophagitis in 1, gastric ulcer in 1 and normal mucosa in 14.
Comparison of group B children with mucosal healing (Marsh < 2) and mucosal injury (Marsh ≥ 2)
Marsh < 2
Marsh ≥ 2
(n = 47)
(n = 6)
Serology [n (%)]
Negativity of all antibody tests (anti-tissue transglutaminase IgA and -IgG, anti-deamidated-gliadin-peptide IgA and -IgG, anti-endomysial antibodies IgA)
Positivity of at least one antibody test
Chief complaint [n (%)]
Weight-for-age z score, mean ± standard deviation
−0.66 ± 1.31
−1.04 ± 2.03
Height-for-age z score, mean ± standard deviation
−0.63 ± 1.21
−1.58 ± 1.74
Good inter-rater reliability among the pathologists was found, kappa coefficients were 0.81 and 0.74 for the biopsies from the bulb and from the descending part of the duodenum, respectively. Before reaching consensus, histological classification differed in 19 children (12.8%); however, in only 2 of these children, classification differences pertained to the presence or absence of mucosal injury (Marsh ≥ 2). The pathologists finally agreed on Marsh ≥ 2 in both children.
No adverse events of endoscopy including biopsies were encountered.
In total, four children (2.7%), all girls, were IgA-deficient. Three girls belonged to group A1, one to group A2.
In this study, we provide evidence that antibody tests are more reliably predicting mucosal status in children with CD before than in children after prescription of a GFD.
In our patients, according to AUCs (Figure 2), TG2-IgA, DPG-IgG and DPG-IgA antibody tests performed very well in diagnosing CD in group A, similar to the performances reported elsewhere . However, when used for monitoring mucosal status in CD after a median of 2.2 years after primary diagnosis (group B), all tests suffered a performance loss turning out to be significant in case of DPG-IgA. According to LRs, additional parameters quantifying the non-invasive tests' performance, TG2-IgA, DGP-IgG and -IgA antibodies were most informative and clinically useful with respect to diagnosing CD in group A. Conversely, the limited ability to detect mucosal injury in group B was reflected by LRs + in all tests being < 10. Regarding LRs-, only negative EMA had an LR- < 0.1 thus being an informative and clinically useful marker of mucosal healing in CD .
Positive EMA, however, were detected in 18 children from group B. Twelve of these EMA-positive children showed mucosal healing, a finding that reflects faster mucosal recovery than EMA-seroconversion. Indeed, EMA-positive children exhibiting mucosal healing had been on the GFD for a significantly shorter period of time than the EMA-negative children. Moreover, 9 of 12 developed EMA-negativity on further follow-up. This delayed seroconversion might partially explain positive EMA in subjects showing mucosal healing . However, adherence to the dietary treatment was not evaluated in this study nor was small intestinal mucosa examined for IgA deposits . Therefore, we cannot rule out that serum EMA-positivity is more sensitive than gross histological damage to detect minor dietary transgressions. Positive EMA without histological evidence of CD were also detected in 3 children from group A2. EMA normalized on a normal gluten-containing diet in 2 of the EMA-positive group B children while the third became seronegative on a self-prescribed GFD and stayed seronegative even after a 11-month gluten challenge. However, since EMA is a very strong predictor of a subsequent CD diagnosis , there is need of further follow-up in these children including endoscopy.
All the children participating in the study underwent EGD as criterion standard for the evaluation of the diagnostic reliability of the antibody tests. In the light of the possibility to diagnose CD without biopsies , an important finding of our study was that experiences with the diagnostic biopsy did not deter two thirds of the children eligible for group B from undergoing re-biopsy. We found mucosal healing in the majority of the re-biopsied children, 90% of them had Marsh < 2. All group B children with mucosal injury were ≥ 9 years old and belonged to the subgroup that had been on a GFD < 2 years. In children, the long-term mucosal healing rate was reported to be 100% and histological recovery might even occur after more than 2 years after primary diagnosis . In contrast, it has been shown that children diagnosed after the age of 4 tend to follow the GFD less strictly and therefore are expected to have a higher prevalence of mucosal injury . Surprisingly, a very low frequency (1.8%) of isolated increase of intraepithelial lymphocyte count (Marsh 1) was found among group B children. This finding could be related to the somewhat high cut-off for an increased intraepithelial lymphocyte count used in the study (≥ 30 lymphocytes/100 epithelial cells).
This study has several strengths. In the first place, it was conducted in children. Concerning the correlation between follow-up histology and serology, most of the studies investigating this correlation were conducted in adults [2, 3, 8, 9, 11, 24–27]. However, in one pediatric study on the value of DGP-antibodies in the follow-up of CD, only 13 children had both re-endoscopy and follow-up serology . Another study with children is of limited value in the aspect that only seroconverted children were included . Then, in contrast to the current study, most of the studies reporting on correlations between follow-up histology and serologies are retrospective [2, 26, 28, 29]. Another strength of the current study was the cut-off point being adjusted for the study population in case of all antibody tests according to ROC analysis (Table 1). This adjustment is especially important in children since manufacturers’ cut-offs are usually based on data from adults. A further advantage was the use of AUCs. AUCs as effective single indicators of the agreement between a test and a reference standard facilitate the comparison of the overall performance between different non-invasive diagnostic tests [15, 16]. Additionally, in order to increase the reliability of the reference standard, two pathologists, before reaching a consensus diagnosis, independently reviewed all biopsy samples, which had been taken according to guidelines [7, 30, 31]. Furthermore, we were able to systematically examine all recommended serologic tests. In contrast to recent recommendations for the use of DGP-IgA in monitoring treated CD , we found that DGP-IgA suffered a significant performance loss when used for follow-up in children. We therefore consider DGP-IgA less reliable for follow-up purposes compared with EMA, TG2-IgA and DGP-IgG. For the comparisons regarding the performance loss of the serologic tests other than DGP-IgA, mean empirical power was rather low, a finding we consider an important limitation of this study. Therefore, future research is needed to further clarify the correlation of EMA, TG2-IgA and DGP-IgG with follow-up histology or identify other reliable non-invasive follow-up tests in CD.
In conclusion, this study demonstrates the limited value of serologic testing in the follow-up of pediatric CD with respect to the mucosal status. Only the normalization of EMA indicates mucosal healing with acceptable accuracy. As long as there is a lack of more reliable tools for non-invasive follow-up, EMA should be used as follow-up tool of first choice. However, more reliable non-invasive follow-up tools would be of great clinical and research utility with respect to the individualization of the GFD strictness and upcoming studies evaluating the efficacy of new CD treatment modalities, respectively.
Deamidated gliadin petide
Area under the curve
Receiver operating characteristic
- Oberhuber G, Granditsch G, Vogelsang H: The histopathology of coeliac disease: time for a standardized report scheme for pathologists. Eur J Gastroenterol Hepatol. 1999, 11 (10): 1185-1194.View ArticlePubMed
- Rubio-Tapia A, Rahim MW, See JA, Lahr BD, Wu TT, Murray JA: Mucosal recovery and mortality in adults with celiac disease after treatment with a gluten-free diet. Am J Gastroenterol. 2010, 105 (6): 1412-1420. 10.1038/ajg.2010.10.PubMed CentralView ArticlePubMed
- Kaukinen K, Peraaho M, Lindfors K, Partanen J, Woolley N, Pikkarainen P, Karvonen AL, Laasanen T, Sievanen H, Maki M, et al: Persistent small bowel mucosal villous atrophy without symptoms in coeliac disease. Aliment Pharmacol Ther. 2007, 25 (10): 1237-1245. 10.1111/j.1365-2036.2007.03311.x.View ArticlePubMed
- Lebwohl B, Granath F, Ekbom A, Smedby KE, Murray JA, Neugut AI, Green PH, Ludvigsson JF: Mucosal healing and risk for lymphoproliferative malignancy in celiac disease: a population-based cohort study. Ann Intern Med. 2013, 159 (3): 169-175. 10.7326/0003-4819-159-3-201308060-00006.PubMed CentralView ArticlePubMed
- Bai JC, Fried M, Corazza GR, Schuppan D, Farthing M, Catassi C, Greco L, Cohen H, Ciacci C, Eliakim R, et al: World gastroenterology organisation global guidelines on celiac disease. J Clin Gastroenterol. 2013, 47 (2): 121-126. 10.1097/MCG.0b013e31827a6f83.View ArticlePubMed
- Husby S, Koletzko S, Korponay-Szabo IR, Mearin ML, Phillips A, Shamir R, Troncone R, Giersiepen K, Branski D, Catassi C, et al: European society for pediatric gastroenterology, hepatology, and nutrition guidelines for the diagnosis of coeliac disease. J Pediatr Gastroenterol Nutr. 2012, 54 (1): 136-160. 10.1097/MPG.0b013e31821a23d0.View ArticlePubMed
- Hill ID, Dirks MH, Liptak GS, Colletti RB, Fasano A, Guandalini S, Hoffenberg EJ, Horvath K, Murray JA, Pivor M, et al: Guideline for the diagnosis and treatment of celiac disease in children: recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition. J Pediatr Gastroenterol Nutr. 2005, 40 (1): 1-19. 10.1097/00005176-200501000-00001.View ArticlePubMed
- Dickey W, Hughes DF, McMillan SA: Disappearance of endomysial antibodies in treated celiac disease does not indicate histological recovery. Am J Gastroenterol. 2000, 95 (3): 712-714. 10.1111/j.1572-0241.2000.01838.x.View ArticlePubMed
- Sategna-Guidetti C, Pulitano R, Grosso S, Ferfoglia G: Serum IgA antiendomysium antibody titers as a marker of intestinal involvement and diet compliance in adult celiac sprue. J Clin Gastroenterol. 1993, 17 (2): 123-127. 10.1097/00004836-199309000-00007.View ArticlePubMed
- Vecsei AK, Graf UB, Vogelsang H: Follow-up of adult celiac patients: which noninvasive test reflects mucosal status most reliably?1. Endoscopy. 2009, 41 (2): 123-128. 10.1055/s-0028-1103484.View ArticlePubMed
- Hopper AD, Hadjivassiliou M, Hurlstone DP, Lobo AJ, McAlindon ME, Egner W, Wild G, Sanders DS: What is the role of serologic testing in celiac disease? A prospective, biopsy-confirmed study with economic analysis. Clin Gastroenterol Hepatol. 2008, 6 (3): 314-320. 10.1016/j.cgh.2007.12.008.View ArticlePubMed
- Veress B, Franzen L, Bodin L, Borch K: Duodenal intraepithelial lymphocyte-count revisited. Scand J Gastroenterol. 2004, 39 (2): 138-144. 10.1080/00365520310007675.View ArticlePubMed
- Conley ME, Notarangelo LD, Etzioni A: Diagnostic criteria for primary immunodeficiencies. Representing PAGID (Pan-American Group for Immunodeficiency) and ESID (European Society for Immunodeficiencies). Clin Immunol. 1999, 93 (3): 190-197. 10.1006/clim.1999.4799.View ArticlePubMed
- Swets JA: Measuring the accuracy of diagnostic systems. Science. 1988, 240 (4857): 1285-1293. 10.1126/science.3287615.View ArticlePubMed
- DeLong ER, DeLong DM, Clarke-Pearson DL: Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics. 1988, 44 (3): 837-845. 10.2307/2531595.View ArticlePubMed
- Hanley JA, McNeil BJ: The meaning and use of the area under a receiver operating characteristic (ROC) curve. Radiology. 1982, 143 (1): 29-36.View ArticlePubMed
- Jaeschke R, Guyatt GH, Sackett DL: Users' guides to the medical literature. III. How to use an article about a diagnostic test. B. What are the results and will they help me in caring for my patients? The Evidence-Based Medicine Working Group. JAMA. 1994, 271 (9): 703-707. 10.1001/jama.1994.03510330081039.View ArticlePubMed
- R Core Team: R: A Language and Environment for Statistical Computing. 2013, Vienna, Austria: R Foundation for Statistical Computing, http://www.R-project.org/,
- Giersiepen K, Lelgemann M, Stuhldreher N, Ronfani L, Husby S, Koletzko S, Korponay-Szabo IR: Accuracy of diagnostic antibody tests for coeliac disease in children: summary of an evidence report. J Pediatr Gastroenterol Nutr. 2012, 54 (2): 229-241. 10.1097/MPG.0b013e318216f2e5.View ArticlePubMed
- Koskinen O, Collin P, Korponay-Szabo I, Salmi T, Iltanen S, Haimila K, Partanen J, Maki M, Kaukinen K: Gluten-dependent small bowel mucosal transglutaminase 2-specific IgA deposits in overt and mild enteropathy coeliac disease. J Pediatr Gastroenterol Nutr. 2008, 47 (4): 436-442.View ArticlePubMed
- Kurppa K, Rasanen T, Collin P, Iltanen S, Huhtala H, Ashorn M, Saavalainen P, Haimila K, Partanen J, Maki M, et al: Endomysial antibodies predict celiac disease irrespective of the titers or clinical presentation. World J Gastroenterol. 2012, 18 (20): 2511-2516. 10.3748/wjg.v18.i20.2511.PubMed CentralView ArticlePubMed
- Wahab PJ, Meijer JW, Mulder CJ: Histologic follow-up of people with celiac disease on a gluten-free diet: slow and incomplete recovery. Am J Clin Pathol. 2002, 118 (3): 459-463. 10.1309/EVXT-851X-WHLC-RLX9.View ArticlePubMed
- Hogberg L, Grodzinsky E, Stenhammar L: Better dietary compliance in patients with coeliac disease diagnosed in early childhood. Scand J Gastroenterol. 2003, 38 (7): 751-754. 10.1080/00365520310003318.View ArticlePubMed
- Selby WS, Painter D, Collins A, Faulkner-Hogg KB, Loblay RH: Persistent mucosal abnormalities in coeliac disease are not related to the ingestion of trace amounts of gluten. Scand J Gastroenterol. 1999, 34 (9): 909-914. 10.1080/003655299750025390.View ArticlePubMed
- Ciacci C, Cirillo M, Cavallaro R, Mazzacca G: Long-term follow-up of celiac adults on gluten-free diet: prevalence and correlates of intestinal damage. Digestion. 2002, 66 (3): 178-185. 10.1159/000066757.View ArticlePubMed
- Lebwohl B, Granath F, Ekbom A, Montgomery SM, Murray JA, Rubio-Tapia A, Green PH, Ludvigsson JF: Mucosal healing and mortality in coeliac disease. Aliment Pharmacol Ther. 2013, 37 (3): 332-339. 10.1111/apt.12164.PubMed CentralView ArticlePubMed
- Sharkey LM, Corbett G, Currie E, Lee J, Sweeney N, Woodward JM: Optimising delivery of care in coeliac disease - comparison of the benefits of repeat biopsy and serological follow-up. Aliment Pharmacol Ther. 2013, 38 (10): 1278-1291. 10.1111/apt.12510.View ArticlePubMed
- Basso D, Guariso G, Fogar P, Meneghel A, Zambon CF, Navaglia F, Greco E, Schiavon S, Rugge M, Plebani M: Antibodies against synthetic deamidated gliadin peptides for celiac disease diagnosis and follow-up in children. Clin Chem. 2009, 55 (1): 150-157.View ArticlePubMed
- Bardella MT, Velio P, Cesana BM, Prampolini L, Casella G, Di Bella C, Lanzini A, Gambarotti M, Bassotti G, Villanacci V: Coeliac disease: a histological follow-up study. Histopathology. 2007, 50 (4): 465-471. 10.1111/j.1365-2559.2007.02621.x.View ArticlePubMed
- Ravelli A, Bolognini S, Gambarotti M, Villanacci V: Variability of histologic lesions in relation to biopsy site in gluten-sensitive enteropathy. Am J Gastroenterol. 2005, 100 (1): 177-185. 10.1111/j.1572-0241.2005.40669.x.View ArticlePubMed
- Arguelles-Grande C, Tennyson CA, Lewis SK, Green PH, Bhagat G: Variability in small bowel histopathology reporting between different pathology practice settings: impact on the diagnosis of coeliac disease. J Clin Pathol. 2012, 65 (3): 242-247. 10.1136/jclinpath-2011-200372.View ArticlePubMed
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/14/28/prepub
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