FibroSURE as a noninvasive marker of liver fibrosis and inflammation in chronic hepatitis B
© Zeremski et al.; licensee BioMed Central Ltd. 2014
Received: 19 March 2014
Accepted: 19 June 2014
Published: 3 July 2014
Noninvasive markers of liver fibrosis have not been extensively studied in patients with chronic hepatitis B virus (HBV) infection. Our aim was to evaluate the capacity of FibroSURE, one of the two noninvasive fibrosis indices commercially available in the United States, to identify HBV infected patients with moderate to severe fibrosis.
Forty-five patients who underwent liver biopsy at a single tertiary care center were prospectively enrolled and had FibroSURE performed within an average interval of 11 days of the biopsy.
Of the 45 patients, 40% were Asian, 40% were African American, and 13% were Caucasian; 27% were co-infected with HIV and 67% had no or mild fibrosis. We found FibroSURE to have moderate capacity to discriminate between patients with moderate to high fibrosis and those with no to mild fibrosis (area under receiver operating characteristic [AUROC] curve = 0.77; 95% confidence interval [CI] [0.61, 0.92]). When we combined the fibrosis score determined by FibroSURE with aspartate aminotransferase (AST) measurements and HIV co-infection status, the discriminatory ability significantly improved reaching an AUROC of 0.90 (95% CI [0.80, 1.00]). FibroSURE also had a good ability to differentiate patients with no or mild from those with moderate to high inflammation (AUROC = 0.83; 95% CI [0.71, 0.95]).
FibroSURE in combination with AST levels has an excellent capacity to identify moderate to high fibrosis stages in chronic HBV-infected patients. These data suggest that FibroSURE may be a useful substitute for liver biopsy in chronic HBV infection.
KeywordsHepatitis B virus Liver biopsy replacement Liver fibrosis assessment Liver histology
Chronic hepatitis B virus (HBV) infection affects 350 to 400 million people worldwide [1, 2]. The disease can progress to cirrhosis and hepatocellular carcinoma in approximately 15% to 40% of patients. The long-term complications are more frequent in patients with active viral replication as well as in those with significant intrahepatic inflammation and fibrosis . Therefore, assessment of liver pathology is very important clinically as it identifies patients most likely to benefit from antiviral treatment.
Currently, the “gold standard” for the assessment of hepatic fibrosis is liver biopsy. Liver biopsy is recommended by the most recent AASLD guidelines on HBV in order to determine the need for antiviral therapy for those with normal liver enzymes who meet treatment criteria based on the viral load [4, 5]. However, the procedure has several drawbacks including morbidity, mortality, significant sampling error, and high cost. It is associated with marked inter- and intra- observer/pathologist variability, and the use of different scoring systems to assess the degree of intrahepatic inflammation and fibrosis further complicates comparisons between pathologists. These limitations are important issues, particularly when patients seek a second opinion . Finally, the invasive nature of the biopsy makes it unsuitable for serial hepatic sampling. In an attempt to overcome these limitations, several indices that incorporate parameters associated with inflammation or fibrogenesis, such as FibroTest/FibroSURE [7, 8], Forns’ index , or the aspartate aminotransferase (AST)/platelet ratio index , have been proposed as noninvasive alternatives. FibroSURE (Laboratory Corporation of America (LabCorp), Burlington, NC) and FIBROSpect II (Prometheus Laboratories, San Diego, CA) are the only two commercially available noninvasive indices used in the United States. Both of these tests have been developed and extensively validated in patients with hepatitis C [11, 12], but their utility in hepatitis B has not been adequately evaluated. In this study, we prospectively assessed the performance characteristics of FibroSURE as a noninvasive test for measuring liver inflammation and fibrosis in patients with chronic hepatitis B infection.
All HBV-infected patients who underwent a liver biopsy at our institution between 2005 and 2012 were prospectively approached and offered participation in this study. We enrolled 45 HBV-infected patients, 12 (26.7%) of whom were co-infected with HIV. Patients with HCV co-infection and other co-existing liver diseases were excluded. The study was approved by the Weill Cornell Medical College institutional review board and was performed in accordance with the Declaration of Helsinki. After signing an informed consent, each patient’s blood was drawn and sent to LabCorp for performance of the FibroSURE. Alanine aminotransferase (ALT) and AST levels, as well as HBV DNA measurements, were extracted from the electronic medical record and were performed, on average, within 1 month (ALT and AST), or within 2 months (HBV DNA), of the liver biopsy or FibroSURE. All liver biopsies were evaluated according to the Scheuer classification  by the same hepatopathologist (RKY).
Statistical analysis was performed using SAS (SAS Institute Inc., Cary, NC, USA) and R (http://www.r-project.org/). Aminotransferase levels were logarithmically transformed for the analysis. Associations between categorical variables were assessed through Fisher’s exact test and logistic regression. For continuous variables, comparisons between groups were performed using Wilcoxon rank-sum or Kruskal-Wallis tests. Diagnostic abilities of FibroSURE were expressed through the estimated area under the receiver operating characteristic (AUROC) curve and by calculation of the respective sensitivity, specificity, positive and negative predictive values estimated for appropriate cut-off values. The AUROC was estimated by the trapezoidal rule and represents the probability that a randomly selected pair of subjects will be classified correctly. In order to estimate the linear combination of the available variables, which detects fibrosis stage and inflammation grade, we used logistic regression. Comparison of the ROC curves was based on the methods described by DeLong et al.. The significance level in all tests (two-sided) was set to 0.05.
Patients (N = 45)
Mean (SD) age, years
Mean ALT (U/L) (range)
Mean AST (U/L) (range)
Mean HBV DNA (IU/ml) (range)
FibroSURE test was performed an average of 11 days preceding or after the liver biopsy. However, in the majority of patients (62%), it was performed on the same day as the liver biopsy.
HIV/HBV co-infected patients had significantly higher fibrosis levels, as estimated by both liver biopsy (p = 0.0099) and FibroSURE (p = 0.0425), compared to HBV mono-infected patients. Although the histologic grade of intrahepatic necroinflammation did not differ significantly depending upon type of infection, co-infected patients had significantly higher FibroSURE inflammatory scores compared to mono-infected patients (p = 0.0341). Of note, HBV/HIV co-infected patients had significantly higher ALT levels compared to HBV mono-infected patients (p = 0.017).
FibroSURE as a predictor of liver fibrosis
Logistic regression analysis on moderate to severe stage of fibrosis
Stage > =2 (n = 15) n (%) or Mean (SD)
Stage < 2 (n = 30) n (%) or Mean (SD)
Univariable logistic regression
Multivariable logistic regression***
Gender, N (%)
Race, N (%)
Grade > 1
Grade < =1*
log10 ALT (U/L)
log10 AST (U/L)
log10 HBV DNA (IU/ml)
FibroSURE as a predictor of liver inflammation
Unlike HCV, in HBV, treatment endpoints include biochemical responses, as indicated by normalization of ALT, and histological responses, as indicated by reduced necroinflammation and reversion of fibrosis . Although liver biopsy has been considered the “gold standard” for the assessment of intrahepatic pathology, the procedure has significant limitations including invasiveness, high cost and poor reproducibility . In the last decade, several non-invasive indices have been developed as liver biopsy alternatives. While indices, such as FibroSURE and Fibrospect, have largely been developed and validated in patients with chronic HCV infection, their use in HBV-infected patients has not been nearly as extensive. Given the therapeutic importance of necroinflammatory activity and fibrosis in HBV infection, a noninvasive index such as FibroSURE might have an important role in the management of the infection.
In the United States, FibroSURE is one of the two commercially available noninvasive indices of hepatic fibrosis and inflammation. FibroSURE is a multicomponent index comprised of six serum markers including α2-macroglobulin, haptoglobin, apolipoprotein A-1, total bilirubin, γ-glutamyltransferase, and ALT that are combined with patients’ age and gender to calculate separate scores for fibrosis and necroinflammatory activity. It is currently marketed for liver histology assessment in chronic HCV-infected patients, and only a few studies evaluated the utility of this test in chronic HBV infection [15–20]. However, in all of these studies, as opposed to performing FibroSURE in a commercial laboratory where the reproducibility of the test is closely monitored and subject to performance measures, serum marker measurements in the prior studies were performed in local laboratories and calculation of the fibrosis score was performed individually by study authors. To our knowledge, the commercially available FibroSURE test has not been evaluated previously in chronic HBV-infected patients from the United States.
Our data showed that FibroSURE had reasonable ability to discriminate between HBV-infected patients with moderate to high fibrosis and those with mild or no fibrosis. Moreover, the performance of FibroSURE test in our HBV-infected patients was very similar to results previously published for patients infected with HCV . The addition of AST and HIV co-infection status to the FibroSURE score significantly improved its discriminatory capacity, with an AUROC of 0.90, making the combination of these three parameters an excellent index for identification of HBV-infected patients with advanced fibrosis. As a marker of intrahepatic inflammation, FibroSURE showed good ability to discriminate between patients with moderate to high inflammation and those with mild or no inflammation. However, its discriminatory ability did not differ significantly from either ALT or AST individually.
Excellent performance characteristics are only the initial attribute required for the adaptation in the clinic of any diagnostic test. In addition, tests must be conveniently obtainable and readily interpretable in the clinical setting with a high degree of reproducibility as demonstrated through laboratory performance characteristics. Reproducibility is particularly important for serial application of a test, such as might be required of FibroSURE if it were to be used as a benchmark for determining treatment outcome or if it were to be performed repeatedly to assess progression of inflammation or fibrosis. We  and others  have previously shown that the commercially available FibroSURE has the utility to identify fibrosis progression in patients with HCV. Whether FibroSURE has similar prognostic significance in HBV infection remains to be determined.
A limitation of this study is the small sample size. Although all HBV-infected patients who underwent a liver biopsy at our institution between 2005 and 2012 were consecutively approached, their number was small and not all who were approached agreed to study participation. However, the racial diversity of our subjects is representative of the U.S. population of HBV-infected individuals. In contradistinction to other countries, HBV infection prevalence in the United States is estimated to be ~2% among Asians and 0.73% among non-Hispanic Blacks, the ethnicities with the highest HBV prevalence in the U.S. . These two groups account for 39% and 31% of individuals, respectively, in the U.S. with chronic HBV infection. The ethnicity of patients in this study (40% Asian, 40% Black) is reflective of that found in the general U.S. population and can be considered a strength of our study population. An additional limitation of the study is the heterogeneous patient population consisting of unequal numbers of HBV mono-infected and HIV/HBV co-infected patients.
In chronic HBV infection, liver biopsy is usually performed in patients with active disease in order to identify those who might be expected to benefit the most from treatment. However, many patients are reluctant to undergo the procedure due to its invasiveness and high costs. Our study shows that performance of FibroSURE in combination with AST levels can be a good substitute for liver biopsy for estimation of hepatic fibrosis in HBV-infected patients. The test can be performed on one tube of blood easily obtained at the point of care. Based upon these results, we believe that the FibroSURE can be used to identify patients in whom HBV treatment should be considered.
We acknowledge Laboratory Corporation of America for defraying the costs of FibroSURE. We also acknowledge the patients for their participation in the study.
- Lai CL, Ratziu V, Yuen MF, Poynard T: Viral hepatitis B. Lancet. 2003, 362 (9401): 2089-2094.View ArticlePubMedGoogle Scholar
- Kuo A, Gish R: Chronic hepatitis B infection. Clin Liver Dis. 2012, 16 (2): 347-369.View ArticlePubMedGoogle Scholar
- Jury E: EASL International Consensus Conference on Hepatitis B. 13–14 September, 2002: Geneva, Switzerland. Consensus statement (short version). J Hepatol. 2003, 38 (4): 533-540.View ArticleGoogle Scholar
- Rockey DC, Caldwell SH, Goodman ZD, Nelson RC, Smith AD: Liver biopsy. Hepatology. 2009, 49 (3): 1017-1044.View ArticlePubMedGoogle Scholar
- Lok AS, McMahon BJ: Chronic hepatitis B: update 2009. Hepatology. 2009, 50 (3): 661-662.View ArticlePubMedGoogle Scholar
- Zeremski M, Talal AH: Noninvasive markers of hepatic fibrosis: Are they ready for prime time in the management of HIV/HCV co-infected patients?. J Hepatol. 2005, 43 (1): 2-5.View ArticlePubMedGoogle Scholar
- Rossi E, Adams L, Prins A, Bulsara M, De Boer B, Garas G, MacQuillan G, Speers D, Jeffrey G: Validation of the FibroTest biochemical markers score in assessing liver fibrosis in hepatitis C patients. Clin Chem. 2003, 49 (3): 450-454.View ArticlePubMedGoogle Scholar
- Imbert-Bismut F, Ratziu V, Pieroni L, Charlotte F, Benhamou Y, Poynard T: Biochemical markers of liver fibrosis in patients with hepatitis C virus infection: a prospective study. Lancet. 2001, 357 (9262): 1069-1075.View ArticlePubMedGoogle Scholar
- Forns X, Ampurdanes S, Llovet JM, Aponte J, Quinto L, Martinez-Bauer E, Bruguera M, Sanchez-Tapias JM, Rodes J: Identification of chronic hepatitis C patients without hepatic fibrosis by a simple predictive model. Hepatology. 2002, 36 (4 Pt 1): 986-992.View ArticlePubMedGoogle Scholar
- Wai CT, Greenson JK, Fontana RJ, Kalbfleisch JD, Marrero JA, Conjeevaram HS, Lok AS: A simple noninvasive index can predict both significant fibrosis and cirrhosis in patients with chronic hepatitis C. Hepatology. 2003, 38 (2): 518-526.View ArticlePubMedGoogle Scholar
- Poynard T, Imbert-Bismut F, Munteanu M, Messous D, Myers RP, Thabut D, Ratziu V, Mercadier A, Benhamou Y, Hainque B: Overview of the diagnostic value of biochemical markers of liver fibrosis (FibroTest, HCV FibroSure) and necrosis (ActiTest) in patients with chronic hepatitis C. Comp Hepatol. 2004, 3 (1): 8-View ArticlePubMedPubMed CentralGoogle Scholar
- Patel K, Gordon SC, Jacobson I, Hezode C, Oh E, Smith KM, Pawlotsky JM, McHutchison JG: Evaluation of a panel of non-invasive serum markers to differentiate mild from moderate-to-advanced liver fibrosis in chronic hepatitis C patients. J Hepatol. 2004, 41 (6): 935-942.View ArticlePubMedGoogle Scholar
- Scheuer PJ: Classification of chronic viral hepatitis: a need for reassessment. J Hepatol. 1991, 13 (3): 372-374.View ArticlePubMedGoogle Scholar
- DeLong ER, DeLong DM, Clarke-Pearson DL: Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics. 1988, 44 (3): 837-845.View ArticlePubMedGoogle Scholar
- Poynard T, Ngo Y, Munteanu M, Thabut D, Ratziu V: Noninvasive Markers of Hepatic Fibrosis in Chronic Hepatitis B. Curr Hepat Rep. 2011, 10 (2): 87-97.View ArticlePubMedPubMed CentralGoogle Scholar
- Mbaye PS, Sarr A, Sire JM, Evra ML, Ba A, Daveiga J, Diallo A, Fall F, Chartier L, Simon F, Vray M: Liver stiffness measurement and biochemical markers in Senegalese chronic hepatitis B patients with normal ALT and high viral load. PLoS ONE. 2011, 6 (7): e22291-View ArticlePubMedPubMed CentralGoogle Scholar
- Bonnard P, Sombie R, Lescure FX, Bougouma A, Guiard-Schmid JB, Poynard T, Cales P, Housset C, Callard P, Le Pendeven C, Drabo J, Carrat F, Pialoux G: Comparison of elastography, serum marker scores, and histology for the assessment of liver fibrosis in hepatitis B virus (HBV)-infected patients in Burkina Faso. Am J Trop Med Hyg. 2010, 82 (3): 454-458.View ArticlePubMedPubMed CentralGoogle Scholar
- Bottero J, Lacombe K, Guechot J, Serfaty L, Miailhes P, Bonnard P, Wendum D, Molina JM, Lascoux-Combe C, Girard PM: Performance of 11 biomarkers for liver fibrosis assessment in HIV/HBV co-infected patients. J Hepatol. 2009, 50 (6): 1074-1083.View ArticlePubMedGoogle Scholar
- Sebastiani G, Vario A, Guido M, Alberti A: Sequential algorithms combining non-invasive markers and biopsy for the assessment of liver fibrosis in chronic hepatitis B. World J Gastroenterol. 2007, 13 (4): 525-531.View ArticlePubMedPubMed CentralGoogle Scholar
- Myers RP, Benhamou Y, Imbert-Bismut F, Thibault V, Bochet M, Charlotte F, Ratziu V, Bricaire F, Katlama C, Poynard T: Serum biochemical markers accurately predict liver fibrosis in HIV and hepatitis C virus co-infected patients. Aids. 2003, 17 (5): 721-725.View ArticlePubMedGoogle Scholar
- Zeremski M, Dimova R, Astemborski J, Thomas DL, Talal AH: CXCL9 and CXCL10 chemokines as predictors of liver fibrosis in a cohort of primarily African-American injection drug users with chronic hepatitis C. J Infect Dis. 2011, 204 (6): 832-836.View ArticlePubMedPubMed CentralGoogle Scholar
- Wilson LE, Torbenson M, Astemborski J, Faruki H, Spoler C, Rai R, Mehta S, Kirk GD, Nelson K, Afdhal N, Thomas DL: Progression of liver fibrosis among injection drug users with chronic hepatitis C. Hepatology. 2006, 43 (4): 788-795.View ArticlePubMedGoogle Scholar
- Ioannou GN: Hepatitis B virus in the United States: infection, exposure, and immunity rates in a nationally representative survey. Ann Intern Med. 2011, 154 (5): 319-328.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/14/118/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.