Association of a probiotic to a Helicobacter pylorieradication regimen does not increase efficacy or decreases the adverse effects of the treatment: a prospective, randomized, double-blind, placebo-controlled study
© Navarro-Rodriguez et al.; licensee BioMed Central Ltd. 2013
Received: 26 June 2012
Accepted: 19 March 2013
Published: 26 March 2013
The treatment for the eradication of Helicobacter pylori (H. pylori) is complex; full effectiveness is rarely achieved and it has many adverse effects. In developing countries, increased resistance to antibiotics and its cost make eradication more difficult. Probiotics can reduce adverse effects and improve the infection treatment efficacy.
If the first-line therapy fails a second-line treatment using tetracycline, furazolidone and proton-pump inhibitors has been effective and low cost in Brazil; however it implies in a lot of adverse effects. The aim of this study was to minimize the adverse effects and increase the eradication rate applying the association of a probiotic compound to second-line therapy regimen.
Patients with peptic ulcer or functional dyspepsia infected by H. pylori were randomized to treatment with the furazolidone, tetracycline and lansoprazole regimen, twice a day for 7 days. In a double-blind study, patients received placebo or a probiotic compound (Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium bifidum and Streptococcus faecium) in capsules, twice a day for 30 days. A symptom questionnaire was administered in day zero, after completion of antibiotic therapy, after the probiotic use and eight weeks after the end of the treatment. Upper digestive endoscopy, histological assessment, rapid urease test and breath test were performed before and eight weeks after eradication treatment.
One hundred and seven patients were enrolled: 21 men with active probiotic and 19 with placebo plus 34 women with active probiotic and 33 with placebo comprising a total of 55 patients with active probiotic and 52 with placebo. Fifty-one patients had peptic ulcer and 56 were diagnosed as functional dyspepsia. The per-protocol eradication rate with active probiotic was 89.8% and with placebo, 85.1% (p = 0.49); per intention to treat, 81.8% and 79.6%, respectively (p = 0.53). The rate of adverse effects at 7 days with the active probiotic was 59.3% and 71.2% with placebo (p = 0.20). At 30 days, it was 44.9% and 60.4%, respectively (p = 0.08).
The use of this probiotic compound compared to placebo in the proposed regimen in Brazilian patients with peptic ulcer or functional dyspepsia showed no significant difference in efficacy or adverse effects.
Current Controlled Trials ISRCTN04714018
KeywordsHelicobacter pylori eradication Probiotic Tetracycline Furazolidone Peptic ulcer Functional dyspepsia Treatment efficacy Adverse effects
The Helicobacter pylori (H. pylori) eradication is a very useful tool in preventing the recurrence of peptic ulcer, in the prevention of gastric cancer and in the treatment of Malt lymphoma and gastritis [1–4]. However, the regimens used to H. pylori eradication are complex and require the combination of at least two antibiotics with acid suppressors that must be administered for several days [5, 6]. Therefore they can lead to many adverse effects , most of them related to the medication but also alterations in intestinal bacterial flora due to antibiotic treatment can occur [8, 9]. Most patients have trouble to adhere to treatment and to purchase the drugs that makes the eradication even more difficult.
Brazil is a densely inhabited developing country with a rate of H. pylori infection ranging from 58 to 80% of the adult population [10–17] and most of the infected population has low socioeconomic level. Due to specific characteristics (antibiotic prophylaxis for surgery and treatment of parasitic infections and or sexually transmitted diseases) the primary resistance to antibiotics used in the eradication of H. pylori is high (e.g., nitroimidazole derivatives) [18–23]. Thus, H. pylori eradication in Brazil should focus on the use of regimens with shorter duration and lower cost using highly effective antibiotics. The treatment of choice is the combination of proton pump inhibitor with clarithromycin and amoxicillin which has an efficacy of 85% .
The regimen that includes tetracycline, furazolidone and omeprazole for 7 days has been used as a second-line treatment, with 75% efficacy ; on the other hand furazolidone results in many adverse effects.
Adverse effects are a common cause of lack of adherence to treatment leading to treatment failure in eradicating H. pylori[6, 26]. Treatment failure results in a higher risk of secondary resistance to antibiotics  and will require the use of new and usually less effective ones, with longer duration, higher cost and complex regimens .
Some adverse effects of treatment are intestinal alterations secondary to changes in the microbiota due to antibiotic use [29, 30]. Probiotics would be an excellent tool in controlling bacterial overgrowth and reduce these effects [31–34]. There is also evidence that probiotics may also inhibit the growth of H. pylori, stimulate an immunological response and reduce the inflammatory effects of infection by bacteria [35–37] increasing the rate of H. pylori eradication [38–40].
This study aimed to detect the rate of eradication efficacy and of side effects in H. pylori treatment with the combination of a probiotic compound and the regimen lansoprazole, furazolidone and tetracycline in a prospective, randomized, double-blind, placebo-controlled study.
Developed in 2007 the treatment design had no previous data in literature for the proposed regimen: association of 7 days of treatment to H. pylori eradication added to 30 days of probiotic, both initiating at the same time. The sample calculation was based on a previous study  with a similar regimen, in which 20 mg of omeprazole was once a day intake, with tetracycline 500 mg and furazolidone 200 mg three times a day, for 7 days. By Fisher's exact test, with unilateral hypothesis, expecting an increase in the eradication rate of 70 to 90% with probiotic use, for a power of 0.80 and a significance level of 0.05, the sample size was 56 patients for each group, active or placebo. For a 34 to 15% reduction in the rate of adverse events with probiotic use, for a power of 0.80 and a significance level of 0.05, the sample size was 60 patients for each group.
Patients infected with H. pylori with a previous diagnosis of peptic ulcer or functional dyspepsia were invited to participate in the study.
Inclusion criteria were: more than 18 years old, no previous treatment for the infection, not having a chronic decompensated disease, no use of anti-inflammatory or antibiotic drugs within 4 weeks prior to enrollment and sign of informed consent.
Exclusion criteria were: patients who were pregnant or breastfeeding, patients over 80 years of age or with a history of gastrointestinal surgery and patients with erosive esophagitis or users of low-dose aspirin. Patients with difficulty to understand the treatment or to report disease symptoms and adverse effects were also excluded.
All patients were followed at the Gastroenterology outpatient clinic of this Hospital and had been previously diagnosed by upper digestive endoscopy. The study was approved by the ethics and research committee of the institution: Ethics Committee for Analysis of Research Projects - CAPPesq – of the Clinical Board of Clinics Hospital, Faculty of Medicine, University of São Paulo.
All patients were underwent carbon-13 labeled urea breath test and underwent upper digestive endoscopy. If a patient was taking proton-pump inhibitors or H2 blockers they discontinued it 10 days before the beginning of the study. The symptomatic use of aluminum hydroxide was allowed until the beginning of treatment. During endoscopy mucosal fragments were collected to perform histological assessment with HE and Giemsa staining and rapid urease test from the gastric antrum and gastric body. Eight weeks after treatment completion patients with peptic ulcer were submitted to the same examinations and those with functional dyspepsia underwent urea breath test for heal monitoring.
The patients received tablets and capsules in adequate amount for 7 days of treatment, administered twice a day: 30 mg of lansoprazole, 500 mg of tetracycline and 200 mg of furazolidone. The probiotic compound consisting of Lactobacillus acidophilus (1.25 × 109 CFUs), Lactobacillus rhamnosus (1.25 × 109 CFUs), Bifidobacterium bifidum (1.25 × 109 CFUs) and Streptococcus faecium (1.25 × 109 CFUs) (Klaire Labs, Reno, NV, USA) was provided in a bottle with 60 capsules and instructions to be stored in the refrigerator and to be used regularly during 30 days (7 days of treatment plus 23 days after cessation of the antibiotic). The placebo probiotic consisting of capsules of acidified milk powder (skim milk biologically acidified by commercial yogurt culture) was also provided at the same amount and with the same instructions.
All patients were taught at the sight of medication by the researcher himself and encouraged to maintain full and regular use of medication considering the benefits of eradicating H. pylori. They were also asked to maintain complete abstinence of alcohol, to hamper smoked foods, chocolate, cheese and eggs and not to use antidepressants to avoid interaction with Monoamine Oxidase Inhibitor-like effects of furazolidone during the treatment.
Randomization and study performance
Randomization was carried out using a list obtained by computer. Patients received their numbers in ascending order according to study enrollment. This number corresponded to the randomized regimen for use of medication with an active or placebo probiotic.
None of the patients knew about the randomization and investigators, blinded to the randomization, followed the treatment and performed all examinations independently. The need to disclose the randomization always resulted in the exclusion of the patient.
Drug use control was performed 7 and 30 days after the delivery of medication by counting the remaining tablets in the blisters and the number of probiotic capsules in the bottle. The use of at least 80% of the tablets was considered appropriate.
Patients with peptic ulcer were considered cured of infection when they had negative treatment control tests: rapid urease test, histological analysis and breath test, performed 60 days after the treatment. For patients with functional dyspepsia, they were considered cured when they had a negative urea breath test.
After treatment completion, up to control tests, patients with severe epigastric pain or heartburn symptoms were allowed to use aluminum hydroxide pills symptomatically. The use of any amount of the antacid agent characterized the intensity of the symptom as severe.
The eradication rates were analyzed per intention to treat and per protocol, with 95% confidence interval (P<0.05).
The Chi-square test method with Pearson’s correction factor was used to compare variables and a p value < 0.05 was considered statistically significant. The homogeneity of the groups was evaluated using the nonparametric Chi-square test.
The distribution of patients in the active probiotic and placebo groups, age and gender, both for ulcer disease and for functional dyspepsia, was also evaluated by the Mann–Whitney test.
The analysis of adverse effect incidence was determined by Pearson Chi-square test and the intensity of adverse events at the 7 and 30 day control visits were evaluated by Mann–Whitney test, using the score obtained from symptom questionnaire.
The frequencies of the variables, the percentage of tests and measurements were carried out using Statistical Package for the Social Sciences, 10.0 Version (SPSS Inc., Chicago, IL, EUA).
Data of included population
Patient allocation in groups of active and placebo probiotic agent
Mean age (years)
One patient in the active probiotic group refused the medication regimen for eradication.
Six patients did not undergo eradication control: 3 due to inappropriate use of the eradication regimen (they were concerned with the adverse effects) and three abandoned the study after the eradication regimen use. They were equally divided in the two groups.
Four patients used the eradication regimen and came for adverse effect control after seven days but then discontinued probiotic use although they returned for eradication control. Three were in the placebo probiotic group and one in the active probiotic group.
Eradication rates in groups of active and placebo probiotic
Active (CI 95%)
Placebo (CI 95%)
p = 0.49
Intention to treat
p = 0.53
There was not statistical difference regarding the efficacy of eradication per-protocol among patients with peptic ulcer (91.1%) and those with dyspepsia (84.3%), with p = 0.31. Moreover per-intention to treat also did not present statistical difference (ulcer = 80.4% dyspeptic = 78.6% p = 0.81).
Eradication rates per protocol in peptic ulcer disease and dyspepsia in groups of active and placebo probiotic
Peptic ulcer (CI 95%)
Dyspepsia (CI 95%)
p = 0.23
p = 0.95
Incidence of adverse effects in groups of active and placebo probiotic at 7 and 30-day visits
Intensity of adverse effects in groups of active and placebo probiotic at 7 and 30 day visits
7 day mean/sum rank
30 day mean/sum rank
The triple treatment regimen used in this study favored the short-term therapy (7 days), convenient dosage (twice a day) and simplicity (3 units) aimed at low cost, easy understanding and greater adherence with antibiotics which in our country show low bacterial resistance. These characteristics make treatment adherence depend more on the adverse effects that treatment complexity when compared to the classic regimen including omeprazole, clarithromycin and amoxicillin .
The regimen used in this study differed from a previously tested treatment  with furazolidone and tetracycline administered 3 × day and the proton-pump inhibitor once a day; in this regimen the drugs were equally administered twice a day for 7 days. In the first regimen the eradication effectiveness per-protocol was 75%. In this study, the per-protocol eradication efficacy was higher: 87.5% (perhaps by greater simplicity of the regimen) and this rate was similar to that obtained in our country, with a regimen that included proton-pump inhibitor, clarithromycin and amoxicillin: 88.8% (per protocol), also a short-duration regimen, preferably used as first-line eradication of H. pylori. The differences between the regimens, PPI + amoxicillin + clarithromycin twice a day for 7 days or PPI once a day + Tetracycline + Furazolidone 3 times a day for 7 days were the incidence of severe adverse effects that was much lower for clarithromycin with amoxicillin (3.7%) than with furazolidone with tetracycline (15%).
This study associated a probiotic compound to the antibiotic regimen targeting a lower rate of the adverse effects. The incidence of adverse effects were higher than expected probably because of the use of a standard symptom questionnaire. Unfortunately although there was a difference in incidence and severity of adverse effects between the active probiotic and placebo groups, no statistical significance was observed.
In the literature studies have observed increase of eradication rate and decrease of adverse effects in H. pylori eradication when a probiotic is associated with the treatment of the infection [39–43]. Ojetti and coworkers  used a single strain of lactobacillus with 1×108 CFUs for 14 days also associated with a triple regimen of eradication (PPI + Levofloxacin + Amoxicillin) with 7 days in duration and obtained both increasing eradication and a reduction in adverse effects. Du and colleagues  also with a 7 days triple eradication regimen (PPI + Amoxicillin + Clarithomycin) also with 14 days treatment with a single strain of bacillus (3×107 CFUs) in patient groups approximately equal to ours also obtained eradication increased and adverse effects decreased.
Others studies achieved a decrease of adverse effects without an increase of eradication rate [34, 44, 46, 47]. Among them, one of Manfredi at al. associated a compound of the four different probiotics and prebiotics for 10 days in a sequential treatment for eradication of H. pylori and observed a reduction of adverse effects, although no increase in eradication.
On the other hand some studies didn’t verified significant benefits in probiotic use [48–50]. Yoon  and coworkers joined a compound of 4 probiotics, for 4 weeks, to a treatment of 14 days second-line regimen for H. pylori eradication, with PPI + amoxicillin + moxifloxacin that did not increase eradication or reduce the adverse effects.
The different results are probably due to the different products used, their different concentrations, probiotic strain, dose and duration of use and also the strain of H. pylori in question, as suggested by Vitor  and Wilhelm .
Considering the fact that the use of a probiotic agent also adds more complexity to the process as it increases treatment duration, the benefit of attaining a lower incidence of adverse effects or higher eradication effectiveness with longer treatment duration is debatable, especially because the difference did not reach statistical significance, as also stated by Medeiros et al. .
However if probiotics can reduce the adverse effects of H. pylori eradication it could enable greater adherence to treatment and could increase the eradication rate by intention to treat.
Thus it is necessary to seek other probiotic combinations or other presentations or other dosages or other treatment duration to achieve these goals.
The probiotic compound used in the present study (Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium bifidum and Streptococcus Faecium), administered for 30 days associated to the H. pylori eradication regimen: Lansoprazole 30 mg, Tetracycline 500 mg and Furazolidone 200 mg administered twice a day for 7 days did not show an increase in bacterial eradication effectiveness or decrease in adverse effects of H. pylori eradication treatment in Brazilian patients with peptic ulcer and functional dyspepsia.
Drugs providing supported from Medley Indústria Farmacêutica, São Paulo, Brazil.
- Coelho LG, Zaterka S: [Second Brazilian Consensus Conference on Helicobacter pylori infection]. Arq Gastroenterol. 2005, 42: 128-132.PubMedGoogle Scholar
- Chey WD, Wong BC: American College of Gastroenterology guideline on the management of Helicobacter pylori infection. Am J Gastroenterol. 2007, 102: 1808-1825. 10.1111/j.1572-0241.2007.01393.x.View ArticlePubMedGoogle Scholar
- Malfertheiner P, Megraud F, O'Morain CA, Atherton J, Axon AT, Bazzoli F, Gensini GF, Gisbert JP, Graham DY, Rokkas T, El-Omar EM, Kuipers EJ: Management of Helicobacter pylori infection–the Maastricht IV/ Florence Consensus Report. Gut. 2012, 61: 646-664. 10.1136/gutjnl-2012-302084.View ArticlePubMedGoogle Scholar
- Talley NJ, Fock KM, Moayyedi P: Gastric Cancer Consensus conference recommends Helicobacter pylori screening and treatment in asymptomatic persons from high-risk populations to prevent gastric cancer. Am J Gastroenterol. 2008, 103: 510-514. 10.1111/j.1572-0241.2008.01819.x.View ArticlePubMedGoogle Scholar
- Gasparetto M, Pescarin M, Guariso G: Helicobacter pylori Eradication Therapy: Current Availabilities. ISRN Gastroenterol. 2012, 2012: 186734-View ArticlePubMedPubMed CentralGoogle Scholar
- Cutler AF, Schubert TT: Patient factors affecting Helicobacter pylori eradication with triple therapy. Am J Gastroenterol. 1993, 88: 505-509.PubMedGoogle Scholar
- Qua CS, Manikam J, Goh KL: Efficacy of 1-week proton pump inhibitor triple therapy as first-line Helicobacter pylori eradication regime in Asian patients: is it still effective 10 years on?. J Dig Dis. 2010, 11: 244-248.PubMedGoogle Scholar
- Periti P, Mazzei T, Mini E, Novelli A: Adverse effects of macrolide antibacterials. Drug Saf. 1993, 9: 346-364. 10.2165/00002018-199309050-00004.View ArticlePubMedGoogle Scholar
- Salvo F, Polimeni G, Moretti U, Conforti A, Leone R, Leoni O, Motola D, Dusi G, Caputi AP: Adverse drug reactions related to amoxicillin alone and in association with clavulanic acid: data from spontaneous reporting in Italy. J Antimicrob Chemother. 2007, 60: 121-126. 10.1093/jac/dkm111.View ArticlePubMedGoogle Scholar
- Porras C, Nodora J, Sexton R, Ferreccio C, Jimenez S, Dominguez RL, Cook P, Anderson G, Morgan DR, Baker LH, Greenberg ER, Herrero R: Epidemiology of Helicobacter pylori infection in six Latin American countries (SWOG Trial S0701). Cancer Causes Control. 2012, Available at http://link.springer.com/content/pdf/10.1007%2Fs10552-012-0117-5 Google Scholar
- Zaterka S, Eisig JN, Chinzon D, Rothstein W: Factors related to Helicobacter pylori prevalence in an adult population in Brazil. Helicobacter. 2007, 12: 82-88.View ArticlePubMedGoogle Scholar
- Escobar-Pardo ML, de Godoy AP, Machado RS, Rodrigues D, Fagundes Neto U, Kawakami E: Prevalence of Helicobacter pylori infection and intestinal parasitosis in children of the Xingu Indian Reservation. J Pediatr (Rio J). 2011, 87: 393-398.Google Scholar
- Miranda AC, Machado RS, Silva EM, Kawakami E: Seroprevalence of Helicobacter pylori infection among children of low socioeconomic level in Sao Paulo. Sao Paulo Med J. 2010, 128: 187-191. 10.1590/S1516-31802010000400002.View ArticlePubMedGoogle Scholar
- Dattoli VC, Veiga RV, da Cunha SS, Pontes-de-Carvalho LC, Barreto ML, Alcantara-Neves NM: Seroprevalence and potential risk factors for Helicobacter pylori infection in Brazilian children. Helicobacter. 2010, 15: 273-278. 10.1111/j.1523-5378.2010.00766.x.View ArticlePubMedPubMed CentralGoogle Scholar
- Rodrigues MN, Queiroz DM, Rodrigues RT, Rocha AM, Braga Neto MB, Braga LL: Helicobacter pylori infection in adults from a poor urban community in northeastern Brazil: demographic, lifestyle and environmental factors. Braz J Infect Dis. 2005, 9: 405-410. 10.1590/S1413-86702005000500008.View ArticlePubMedGoogle Scholar
- Rodrigues MN, Queiroz DM, Rodrigues RT, Rocha AM, Luz CR, Braga LL: Prevalence of Helicobacter pylori infection in Fortaleza, Northeastern Brazil. Rev Saude Publica. 2005, 39: 847-849. 10.1590/S0034-89102005000500022.View ArticlePubMedGoogle Scholar
- Almeida Cunha RP, Alves FP, Rocha AM, Rocha GA, Camargo LM, Nogueira PO, Camargo EP, Queiroz DM: Prevalence and risk factors associated with Helicobacter pylori infection in native populations from Brazilian Western Amazon. Trans R Soc Trop Med Hyg. 2003, 97: 382-386. 10.1016/S0035-9203(03)90063-0.View ArticlePubMedGoogle Scholar
- Prazeres Magalhaes P, De Magalhaes Queiroz DM, Campos Barbosa DV, Aguiar Rocha G, Nogueira Mendes E, Santos A, Valle Correa PR, Camargos Rocha AM, Martins Teixeira L, Affonso de Oliveira C: Helicobacter pylori primary resistance to metronidazole and clarithromycin in Brazil. Antimicrob Agents Chemother. 2002, 46: 2021-2023. 10.1128/AAC.46.6.2021-2023.2002.View ArticlePubMedPubMed CentralGoogle Scholar
- Oleastro M, Cabral J, Ramalho PM, Lemos PS, Paixao E, Benoliel J, Santos A, Lopes AI: Primary antibiotic resistance of Helicobacter pylori strains isolated from Portuguese children: a prospective multicentre study over a 10 year period. J Antimicrob Chemother. 2011, 66: 2308-2311. 10.1093/jac/dkr293.View ArticlePubMedGoogle Scholar
- Goh KL, Navaratnam P: High Helicobacter pylori resistance to metronidazole but zero or low resistance to clarithromycin, levofloxacin, and other antibiotics in Malaysia. Helicobacter. 2011, 16: 241-245. 10.1111/j.1523-5378.2011.00841.x.View ArticlePubMedGoogle Scholar
- De Francesco V, Giorgio F, Hassan C, Manes G, Vannella L, Panella C, Ierardi E, Zullo A: Worldwide H. pylori antibiotic resistance: a systematic review. J Gastrointestin Liver Dis. 2010, 19: 409-414.PubMedGoogle Scholar
- Mendonca S, Ecclissato C, Sartori MS, Godoy AP, Guerzoni RA, Degger M, Pedrazzoli J: Prevalence of Helicobacter pylori resistance to metronidazole, clarithromycin, amoxicillin, tetracycline, and furazolidone in Brazil. Helicobacter. 2000, 5: 79-83. 10.1046/j.1523-5378.2000.00011.x.View ArticlePubMedGoogle Scholar
- Godoy AP, Ribeiro ML, Benvengo YH, Vitiello L, Miranda Mde C, Mendonca S, Pedrazzoli J: Analysis of antimicrobial susceptibility and virulence factors in Helicobacter pylori clinical isolates. BMC Gastroenterol. 2003, 3: 20-10.1186/1471-230X-3-20.View ArticlePubMedPubMed CentralGoogle Scholar
- Felga G, Silva FM, Barbuti RC, Navarro-Rodriguez T, Zaterka S, Eisig JN: Clarithromycin-based triple therapy for Helicobacter pylori treatment in peptic ulcer patients. J Infect Dev Ctries. 2010, 4: 712-716.View ArticlePubMedGoogle Scholar
- Silva FM, Eisig JN, Chehter EZ, Silva JJ, Laudanna AA: Omeprazole, furazolidone, and tetracycline: an eradication treatment for resistant H. pylori in Brazilian patients with peptic ulcer disease. Rev Hosp Clin Fac Med Sao Paulo. 2002, 57: 205-208.PubMedGoogle Scholar
- Glupczynski Y, Burette A: Drug therapy for Helicobacter pylori infection: problems and pitfalls. Am J Gastroenterol. 1990, 85: 1545-1551.PubMedGoogle Scholar
- Peitz U, Sulliga M, Wolle K, Leodolter A, Von Arnim U, Kahl S, Stolte M, Borsch G, Labenz J, Malfertheiner P: High rate of post-therapeutic resistance after failure of macrolide-nitroimidazole triple therapy to cure Helicobacter pylori infection: impact of two second-line therapies in a randomized study. Aliment Pharmacol Ther. 2002, 16: 315-324. 10.1046/j.1365-2036.2002.01173.x.View ArticlePubMedGoogle Scholar
- Selgrad M, Malfertheiner P: Treatment of Helicobacter pylori. Curr Opin Gastroenterol. 2011, 27: 565-570. 10.1097/MOG.0b013e32834bb818.View ArticlePubMedGoogle Scholar
- Pérez-Cobas AE, Gosalbes MJ, Friedrichs A, Knecht H, Artacho A, Eismann K, Otto W, Rojo D, Bargiela R, von Bergen M, Neulinger SC, Däumer C, Heinsen FA, Latorre A, Barbas C, Seifert J, Dos Santos VM, Ott SJ, Ferrer M, Moya A: Gut microbiota disturbance during antibiotic therapy: a multi-omic approach. Gut. 2012, Available at http://gut.bmj.com/content/early/2012/12/11/gutjnl-2012-303184.long Google Scholar
- Danese S, Armuzzi A, Romano A, Cremonini F, Candelli M, Franceschi F, Ojetti V, Venuti A, Pola P, Gasbarrini G: Efficacy and tolerability of antibiotics in patients undergoing H. pylori eradication. Hepatogastroenterology. 2001, 48: 465-467.PubMedGoogle Scholar
- Armuzzi A, Cremonini F, Bartolozzi F, Canducci F, Candelli M, Ojetti V, Cammarota G, Anti M, De Lorenzo A, Pola P: The effect of oral administration of Lactobacillus GG on antibiotic-associated gastrointestinal side-effects during Helicobacter pylori eradication therapy. Aliment Pharmacol Ther. 2001, 15: 163-169. 10.1046/j.1365-2036.2001.00923.x.View ArticlePubMedGoogle Scholar
- Cremonini F, Di Caro S, Covino M, Armuzzi A, Gabrielli M, Santarelli L, Nista EC, Cammarota G, Gasbarrini G, Gasbarrini A: Effect of different probiotic preparations on anti-Helicobacter pylori therapy-related side effects: a parallel group, triple blind, placebo-controlled study. Am J Gastroenterol. 2002, 97: 2744-2749. 10.1111/j.1572-0241.2002.07063.x.View ArticlePubMedGoogle Scholar
- Wilhelm SM, Johnson JL, Kale-Pradhan PB: Treating bugs with bugs: the role of probiotics as adjunctive therapy for Helicobacter pylori. Ann Pharmacother. 2011, 45: 960-966. 10.1345/aph.1Q104.View ArticlePubMedGoogle Scholar
- Yasar B, Abut E, Kayadibi H, Toros B, Sezikli M, Akkan Z, Keskin O, Ovunc Kurdas O: Efficacy of probiotics in Helicobacter pylori eradication therapy. Turk J Gastroenterol. 2011, 21: 212-217.View ArticleGoogle Scholar
- Yang YJ, Sheu BS: Probiotics-containing yogurts suppress Helicobacter pylori load and modify immune response and intestinal microbiota in the Helicobacter pylori-infected children. Helicobacter. 2012, 17: 297-304. 10.1111/j.1523-5378.2012.00941.x.View ArticlePubMedGoogle Scholar
- Zhou C, Ma FZ, Deng XJ, Yuan H, Ma HS: Lactobacilli inhibit interleukin-8 production induced by Helicobacter pylori lipopolysaccharide-activated Toll-like receptor 4. World J Gastroenterol. 2008, 14: 5090-5095. 10.3748/wjg.14.5090.View ArticlePubMedPubMed CentralGoogle Scholar
- Hsieh PS, Tsai YC, Chen YC, Teh SF, Ou CM, King VA: Eradication of Helicobacter pylori infection by the probiotic strains Lactobacillus johnsonii MH-68 and L. salivarius ssp. salicinius AP-32. Helicobacter. 2012, 17: 466-477. 10.1111/j.1523-5378.2012.00992.x.View ArticlePubMedGoogle Scholar
- Vítor JM, Vale FF: Alternative therapies for Helicobacter pylori: probiotics and phytomedicine. FEMS Immunol Med Microbiol. 2011, 63: 153-164. 10.1111/j.1574-695X.2011.00865.x.View ArticlePubMedGoogle Scholar
- Canducci F, Armuzzi A, Cremonini F, Cammarota G, Bartolozzi F, Pola P, Gasbarrini G, Gasbarrini A: A lyophilized and inactivated culture of Lactobacillus acidophilus increases Helicobacter pylori eradication rates. Aliment Pharmacol Ther. 2000, 14: 1625-1629. 10.1046/j.1365-2036.2000.00885.x.View ArticlePubMedGoogle Scholar
- Szajewska H, Horvath A, Piwowarczyk A: Meta-analysis: the effects of Saccharomyces boulardii supplementation on Helicobacter pylori eradication rates and side effects during treatment. Aliment Pharmacol Ther. 2010, 32: 1069-1079. 10.1111/j.1365-2036.2010.04457.x.View ArticlePubMedGoogle Scholar
- Zou J, Dong J, Yu X: Meta-analysis: Lactobacillus containing quadruple therapy versus standard triple first-line therapy for Helicobacter pylori eradication. Helicobacter. 2009, 14: 97-107.PubMedGoogle Scholar
- Scaccianoce G, Zullo A, Hassan C, Gentili F, Cristofari F, Cardinale V, Gigliotti F, Piglionica D, Morini S: Triple therapies plus different probiotics for Helicobacter pylori eradication. Eur Rev Med Pharmacol Sci. 2008, 12: 251-256.PubMedGoogle Scholar
- Wang ZH, Gao QY, Fang JY: Meta-Analysis of the Efficacy and Safety of Lactobacillus-containing and Bifidobacterium-containing Probiotic Compound Preparation in Helicobacter pylori Eradication Therapy. J Clin Gastroenterol. 2013, 47: 25-32. 10.1097/MCG.0b013e318266f6cf.View ArticlePubMedGoogle Scholar
- Ojetti V, Bruno G, Ainora ME, Gigante G, Rizzo G, Roccarina D, Gasbarrini A: Impact of Lactobacillus reuteri Supplementation on Anti-Helicobacter pylori Levofloxacin-Based Second-Line Therapy. Gastroenterol Res Pract. 2012, 2012: 740381-View ArticlePubMedPubMed CentralGoogle Scholar
- Du YQ, Su T, Fan JG, Lu YX, Zheng P, Li XH, Guo CY, Xu P, Gong YF, Li ZS: Adjuvant probiotics improve the eradication effect of triple therapy for Helicobacter pylori infection. World J Gastroenterol. 2012, 18: 6302-6307. 10.3748/wjg.v18.i43.6302.View ArticlePubMedPubMed CentralGoogle Scholar
- Manfredi M, Bizzarri B, Sacchero RI, Maccari S, Calabrese L, Fabbian F, De'Angelis GL: Helicobacter pylori infection in clinical practice: probiotics and a combination of probiotics + lactoferrin improve compliance, but not eradication, in sequential therapy. Helicobacter. 2012, 17: 254-263. 10.1111/j.1523-5378.2012.00944.x.View ArticlePubMedGoogle Scholar
- Tolone S, Pellino V, Vitaliti G, Lanzafame A, Tolone C: Evaluation of Helicobacter Pylori eradication in pediatric patients by triple therapy plus lactoferrin and probiotics compared to triple therapy alone. Ital J Pediatr. 2012, 38: 63-10.1186/1824-7288-38-63.View ArticlePubMedPubMed CentralGoogle Scholar
- Cats A, Kuipers EJ, Bosschaert MA, Pot RG, Vandenbroucke-Grauls CM, Kusters JG: Effect of frequent consumption of a Lactobacillus casei-containing milk drink in Helicobacter pylori-colonized subjects. Aliment Pharmacol Ther. 2003, 17: 429-435. 10.1046/j.1365-2036.2003.01452.x.View ArticlePubMedGoogle Scholar
- Yoon H, Kim N, Kim JY, Park SY, Park JH, Jung HC, Song IS: Effects of multistrain probiotic-containing yogurt on second-line triple therapy for Helicobacter pylori infection. J Gastroenterol Hepatol. 2011, 26: 44-48.View ArticlePubMedGoogle Scholar
- Kindermann A, Lopes AI: Helicobacter pylori infection in pediatrics. Helicobacter. 2009, 14 (Suppl 1): 52-57.View ArticlePubMedGoogle Scholar
- Medeiros JA, Goncalves TM, Boyanova L, Pereira MI, de Carvalho JN, Pereira AM, Cabrita AM: Evaluation of Helicobacter pylori eradication by triple therapy plus Lactobacillus acidophilus compared to triple therapy alone. Eur J Clin Microbiol Infect Dis. 2011, 30: 555-559. 10.1007/s10096-010-1119-4.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/13/56/prepub
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