2D ultrasonography and contrast enhanced ultrasound for the evaluation of cavitating mesenteric lymph node syndrome in a patient with refractory celiac disease and enteropathy T cell lymphoma
- Cristina Pojoga†1,
- Lidia Ciobanu†1, 2,
- Alexandru Florin Badea2,
- Emil Boţan1,
- Cosmin Caraiani1,
- Claudia Hagiu1, 2,
- Grigore Băciuţ1, 2 and
- Radu Badea1, 2Email author
© Pojoga et al.; licensee BioMed Central Ltd. 2013
Received: 15 July 2012
Accepted: 6 February 2013
Published: 11 February 2013
The cavitating mesenteric lymph node syndrome (CMLNS) is a rare manifestation of celiac disease, with an estimated mortality rate of 50%. Specific infections and malignant lymphoma may complicate its clinical course and contribute to its poor prognosis. Diagnosing the underlying cause of CMLNS can be challenging. This is the first report on contrast enhanced ultrasound (CEUS) findings in enteropathy associated T-cell lymphoma (EATL) complicating CMLNS in a gluten-free compliant patient with persistent symptoms and poor outcome.
We present the case of a 51-year old Caucasian male patient, diagnosed with celiac disease and CMLNS. Despite his compliance to the gluten-free diet the symptoms persisted and we eventually considered the possible development of malignancy. No mucosal changes suggestive of lymphoma were identified with capsule endoscopy. Low attenuation mesenteric lymphadenopathy, without enlarged small bowel segments were seen on computed tomography. CEUS revealed arterial rim enhancement around the necrotic mesenteric lymph nodes, without venous wash-out. No malignant cells were identified on laparoscopic mesenteric lymph nodes biopsies. The patient died due to fulminant liver failure 14 months later; the histopathological examination revealed CD3/CD30-positive atypical T-cell lymphocytes in the liver, mesenteric tissue, spleen, gastric wall, kidney, lung and bone marrow samples; no malignant cells were present in the small bowel samples.
CEUS findings in EATL complicating CMLNS include arterial rim enhancement of the mesenteric tissue around the cavitating lymph nodes, without venous wash-out. This vascular pattern is not suggestive for neoangiogenesis, as arteriovenous shunts from malignant tissues are responsible for rapid venous wash-out of the contrast agent. CEUS failed to provide a diagnosis in this case.
KeywordsCEUS Celiac disease Peripheral T-cell lymphoma Cavitating mesenteric lymph node syndrome
Cavitating mesenteric lymph node syndrome (CMLNS) is a rare, poorly understood complication of celiac disease characterized by central necrosis of mesenteric lymph nodes . Multiple areas of necrotizing cavitation in reticulo-endothelial tissues are frequently associated with splenic hypo-function, the presence of Howell-Jolly bodies, monocytosis, lymphocytosis and increased platelet count [1, 2]. The estimated mortality rate is 50% . Enteropathy associated T cell lymphoma (EATL) is a possible complication of the necrosis of mesenteric lymph nodes [2, 3]. Hepatic failure is a rare complication of celiac disease of unknown pathogenesis; the predisposition to autoimmunity may, in part, explain the involvement of the liver . In patients associating CMLNS, septicemia or malignant T-lymphocyte infiltration of the liver may explain the liver insufficiency [2, 3].
Diagnosing the underlying condition of the cavitating mesenteric lymph node syndrome (either infectious or malignant) can be challenging; a lymphoma can still be notoriously difficult to diagnose, despite multiple biopsies . Even lymphomas with T-cell immunophenotypic features have been reported in extra intestinal sites (liver, spleen), complicating celiac disease, but without intestinal involvement .
Computed tomography (CT) or magnetic resonance imaging (MRI) can detect fat-fluid levels in mesenteric cystic masses, considered as specific findings for CMLNS [6, 7]; furthermore, these techniques can identify a segmental enlargement of the small bowel, suggestive for lymphoma. More recently developed, contrast enhanced ultrasound (CEUS) can depict the blood flow in small vessels, under 40 microns, useful to describe the tumor angiogenesis patterning . The intense, anarchic arterial enhancement with rapid venous wash-out of the contrast agent is highly suggestive for malignancy, since the malignant tissue is characterized by numerous arteriovenous shunts .
We report a case of celiac disease complicated with CMLNS, with persisting symptoms despite adherence to the gluten-free diet. The imaging and histological examinations failed to document the presence of malignancy during the patient’s lifetime. A fulminant liver failure caused the patient’s death 14 months after the initial diagnosis. The postmortem histopathological examination revealed abnormal T-cell lymphocytes in the liver, mesenteric tissue, spleen, gastric walls, kidney, lungs and bone marrow; no malignant cells were present in the examined small bowel samples.
Three months later, the patient complained of persistent symptoms. The stool examination revealed a Yersinia enterocolitica infection. The patient received adequate antibiotic therapy, resulting in stool sterilization.
After another five months, the patient presented with fever (39°Celsius) and severe liver failure. The blood cultures were negative. The serological markers for viral or autoimmune hepatitis and leptospirosis were also negative. The already severe clinical status worsened, with the development of hepatic encephalopathy and severe upper gastrointestinal bleeding. Despite intensive supportive treatment, the patient died 48 hours after admission.
The pathogenesis of CMLNS is not fully understood at the moment. One of the proposed theories involved an excessive antigenic exposure of the immune system via a damaged intestinal mucosa, leading to depletion of cellular lymphoid elements in the mesenteric lymph nodes and spleen, causing a cystic change or ‘cavitation’ in some patients with celiac disease. An alternative hypothesis is the necrosis of mesenteric lymph nodes triggered by localized immune-mediated complement activation and intravascular coagulation . There were several reports of lymph node necrosis associated with intestinal infection with Mycobacterium spp, Yersinia spp  or Tropheryma whippelii . Our patient had an infection with Yersinia enterocolitica during the course of his illness. However, this infection cannot be regarded as the cause of CMLNS, because the symptoms persisted even after adequate antibiotic treatment leading to stool sterilization.
Half of the CMLNS patients have a poor prognosis, but some reports mentioned a good outcome if a strict gluten-free diet was observed [10, 11]. In celiac disease, however, a malignant lymphoma may also be the cause of mesenteric lymph node necrosis, being in part responsible for the poor prognosis .
The diagnosed of CMLNS is based on imaging and typical histopathological examination. The necrotizing mesenteric lymph nodes are described as anechoic cysts on an abdominal ultrasound. Computed tomography studies reveal central low attenuation with enhancing rims . Sometimes fat-fluid levels within the masses may be apparent on CT ; this feature is considered unique to the cavitated mesenteric adenopathies associated with celiac disease. The specific fat-fluid levels were also found in one CMLNS case on an MRI examination .
The diagnosis of complicated cavitating mesenteric lymph node syndrome (either infectious or malignant) can be challenging; a lymphoma is still notoriously difficult to diagnose, despite multiple biopsies . Lymphomas with T-cell immunophenotypic features have been reported in extra intestinal sites (liver, spleen), as a complication of celiac disease, but without intestinal involvement . In our case, the initial biopsies did not reveal abnormal T-cell lymphocytes, but the virtual lack of immunopositivity for CD8 intraepitelial lymphocytes might have been suggestive of a diagnosis of refractory celiac disease type II . Also the presence of isolated large CD30+ lymphocytes in the lamina propria in early biopsies could have represented minimal infiltration by the patient’s lymphoma .
The capsule endoscopy and CT did not find any small bowel mucosal changes suggestive of enteral lymphoma and no other small bowel biopsies were taken. The contrast-enhanced ultrasound was performed in order to obtain more specific information about the vascular pattern of the lymph node “walls” and the surrounding mesenteric tissue.
The assessment of neovascularization by CEUS is based on its ability to depict the blood flow in small vessels. In malignant tumors a rapid and intense enhancement is seen in the arterial phase, with rapid wash-out of the contrast agent in the venous phase. This vascular pattern is explained by arteriovenous shunts. The timing of hypo enhancing on CEUS may be correlated with tumor cell differentiation; well-differentiated tumors wash out more slowly than poorly differentiated ones . The quantitative CEUS parameters in assessing neoangiogenesis processes were documented on hepatocarcinoma, ovarian and breast malignant tumors [15–17]. In malignant lymph nodes, CEUS depicts vessels penetrating the node’s capsule away from the hilum; reactive adenopathies have a singular vascular pedicle at the hilum with regular branches towards the periphery. Based on these findings, CEUS can improve the results of Doppler ultrasound in the differential diagnosis of lymph nodes with a sensitivity, specificity and accuracy rate of up to 84%, 79% and 80% respectively . These studies were performed on superficial lymph nodes. However, in several studies, lymphomas seem to have a benign vascular pattern [19, 20]. To date there is not enough evidence for the accuracy of CEUS in assessing different types of lymphoma . In our case, intense enhancement was observed in the arterial phase in the surrounding mesenteric tissue of necrotic lymph nodes, without rapid vascular washout in the venous phase, but it was not suggestive of malignancy. CEUS failed to provide a diagnosis of tumor neoangiogenesis in this case. This lack of venous wash-out may be due to arborizing venules found in some peripheral T-cell lymphomas .
In this case, 18 F-FDG PET scan could have detected early EATL; previous studies documented its role in patients with refractory celiac disease, being more sensitive than CT in detecting sites affected by lymphoma . This investigation was not available in our institution.
In patients associating CMLNS, liver failure may be due to septicemia or to malignant T-lymphocyte infiltration of the liver [2, 3]. In our case, the microscopic examination revealed abnormal T-cell lymphocytes in the liver, spleen, mesenteric tissue, gastric walls, kidney, lung and bone marrow. No malignant cells were observed in the small bowel samples examined. It is possible that the EATL was unsampled, despite multiple biopsies, as it was previous reported . Other authors described the same features of a rare peripheral T-cell lymphoma, associated with celiac disease, characterized by a rearrangement of the gamma-delta T-cell receptor, responsible for the aggressiveness of this tumor [2, 24]. T-cell receptor PCR or flow cytometry were not performed in our case.
This is the first report of a CEUS examination in CMLNS complicated with EATL that revealed an arterial rim enhancement around necrotic lymph nodes without venous wash-out. As this vascular pattern is not suggestive for tumor neoangiogenesis, the investigation failed to provide a diagnosis on this case.
The consent for publication was signed by the patient’s son.
Cavitating mesenteric lymph node syndrome
Contrast enhanced ultrasonography
Enteropathy-associated T cell lymphoma
Magnetic resonance imaging.
We thank Ana Stefanescu for English language supervising, on behalf of Ultrasound Department and Molnar Gheza who performed the diagnostic laparoscopy.
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