Systemic administration of a novel human umbilical cord mesenchymal stem cells population accelerates the resolution of acute liver injury
© Burra et al.; licensee BioMed Central Ltd. 2012
Received: 21 November 2011
Accepted: 12 July 2012
Published: 12 July 2012
Hepatocytes and stem cells transplantation may be an alternative to liver transplantation in acute or chronic liver disease. We aimed to evaluate the therapeutic potential of mesenchymal stem cells from human umbilical cord (UCMSCs), a readily available source of mesenchymal stem cells, in the CCl4-induced acute liver injury model.
Mesenchymal stem cells profile was analyzed by flow cytometry. In order to evaluate the capability of our UCMSCs to differentiate in hepatocytes, cells were seeded on three different supports, untreated plastic support, MatrigelTM and human liver acellular matrix. Cells were analyzed by immunocitochemistry for alpha-fetoprotein and albumin expression, qPCR for hepatocyte markers gene expression, Periodic Acid-Schiff staining for glycogen storage, ELISA for albumin detection and colorimetric assay for urea secretion.
To assess the effects of undifferentiated UCMSCs in hepatic regeneration after an acute liver injury, we transplanted them via tail vein in mice injected intraperitoneally with a single dose of CCl4. Livers were analyzed by histological evaluation for damage quantification, immunostaining for Kupffer and stellate cells/liver myofibroblasts activation and for UCMSCs homing. Pro- and anti-inflammatory cytokines gene expression was evaluated by qPCR analysis and antioxidant enzyme activity was measured by catalase quantification.
Data were analyzed by Mann–Whitney U-test, Kruskal-Wallis test and Cuzick’s test followed by Bonferroni correction for multiple comparisons.
We have standardized the isolation procedure to obtain a cell population with hepatogenic properties prior to in vivo transplantation. When subjected to hepatogenic differentiation on untreated plastic support, UCMSCs differentiated in hepatocyte-like cells as demonstrated by their morphology, progressive up-regulation of mature hepatocyte markers, glycogen storage, albumin and urea secretion. However, cells seeded on 3D-supports showed a minor or negligible differentiation capacity.
UCMSCs-transplanted mice showed a more rapid damage resolution, as shown by histological analysis, with a lower inflammation level and an increased catalase activity compared to CCl4-treated mice.
Our findings show that UCMSCs can be reliably isolated, have hepatogenic properties and following systemic administration are able to accelerate the resolution of an acute liver injury without any differentiation and manipulation. These features make UCMSCs strong candidates for future application in regenerative medicine for human acute liver disease.
KeywordsMesenchymal stem cells Umbilical cord Hepatocyte-like cells Cell transplantation Acute liver injury Regenerative medicine
Transplantation is the gold standard procedure for treating acute and chronic end-stage liver disease , but the shortage of available organs makes it mandatory to seek alternative therapeutic strategies. Replacing diseased hepatocytes and stimulating endogenous and exogenous regeneration by stem cells represent the main aims of liver-oriented cell therapy [2, 3].
Recent developments in stem cell technology have raised the hopes of identifying new expandable sources of liver cells for use in regenerative medicine  and prompted studies on the best support for their growth. Embryonic stem cells can be considered the best model of multipotency, but their use is limited due to legal issues, in Italy at least (L. n. 40/2004), as well as safety and ethical concerns . Adult stem cells have consequently been widely explored in recent years as a more acceptable source of cells, including the mesenchymal stem cells (MSCs), a population of multipotent progenitors capable of differentiating towards adipogenic, osteogenic , and hepatogenic lineages [7, 8] with a low immunogenicity . Therefore this cell population is considered to be a promising candidate for novel cell-based therapeutic strategies .
Bone marrow is considered the main source of MSCs , but their number decreases significantly with age [12, 13] and this has led to the evaluation of alternative sources such as adipose tissue  and embryo-derived tissues, e.g. placenta , amniotic fluid , umbilical cord blood (UCB)  and umbilical cord (UC) .
UC cells obtained from the sub-endothelial layer of the umbilical vein can differentiate in vitro into adipocytes and osteoblasts [19, 20], and - when isolated from umbilical cord jelly - they can also differentiate in vitro and in vivo into a myogenic lineage, as previously reported by our group , confirming the presence of plasticity in this population of foetal-derived tissues.
MSC transplantation has been explored as a new clinical approach to repair injured tissue. Following systemic administration MSCs are recruited in the area of ischemia or injury, as was demonstrated in lung , heart  and kidney . So far, the possibility of using human UCMSCs to repair acute liver damage has not been evaluated.
Therefore, the main aim of this study was to evaluate the therapeutic potential of adult mesenchymal stem cells from human umbilical cord (UCMSCs) in a murine model of acute liver injury using carbon tetrachloride (CCl4), a potent hepatotoxic chemical.
More than one protocol has been proposed to isolate these cells without reaching a scientific agreement. It is clearly fundamental to standardize the isolation procedure to obtain an adequate cell population with hepatogenic properties prior to performing successful in vivo transplantation. To investigate these hepatogenic capacities we have induced UCMSCs differentiation towards hepatic lineages in vitro. Since hepatocytes are known to lose their specific functions rapidly when cultured on a conventional support  we sought the best cell support for hepatogenic differentiation.
Stem cell differentiation can be stimulated by growth factors and extracellular matrix (ECM) components are used as a cell culture support. Using an homologous acellular matrix derived from surgical specimens represents an interesting tissue engineering approach since the matrix is biocompatible, contains adhesion molecules and growth factors, and it is obtained from a healthy organ [26, 27] and not from hepatoma cell lines, e.g. Matrigel™ .
Therefore, we induced UCMSCs hepatic differentiation seeded them on MatrigelTM, human liver acellular matrix and on classic petri dishes.
UCMSCs isolation and characterization
Furthermore, RT-PCR evaluation on UCMSCs obtained from newborn males (representing 53% of our samples) showed the SRY gene expression, confirming the foetal origin of the isolated cells, as previously reported by our group .
In vitroUCMSCs hepatogenic differentiation on untreated plastic support
The expression of specific hepatic markers such as the immature hepatoblast marker alpha-fetoprotein (AFP) and the mature hepatocyte markers albumin, microsomal trialcylglycerol transfer protein (MTP) and tryptophan 2–3 dioxygenase (TDO) mRNAs was assessed by qPCR. Unexpectedly, UCMSCs constitutively expressed the messengers coding for hepatic markers generally attributed to mature hepatocytes, suggesting their hepatogenic potentiality.
qPCR analysis for hepatic markers in differentiated UCMSCs
(z = −2.08)
(z = 2.30)
(z = 3.08)
(z = 3.12)
(z = 2.72)
(z = 2.51)
In fact, the expression of AFP in differentiating conditions was significantly lower than in control cells at each time point (*p values shown in Table 1). This down-regulation was significant during the time course (¥p = 0.0378 z = −2.08).
In contrast, the expression of albumin mRNA was significantly increased at each time point (*p values reported in Table 1) and this up-regulation was time dependent (¥p = 0.0219 z = 2.30).
Levels of MTP mRNA were significantly up-regulated only from the 14th day onwards and this increase was time-dependent, as confirmed by Cuzick’s trend test (¥p = 0.0021 z = 3.08). TDO (an enzyme belonging to the family of oxidoreductases) mRNA expression was significantly up-regulated at each time point in a time-dependent manner (¥p = 0.0023 z = 3.12) (Table 1).
Immunocytochemistry performed using specific anti-human albumin antibodies clearly demonstrated the presence of albumin already after 14 days of differentiation (while the protein was never detected in control cells). No AFP expression was detectable in either the differentiated or the control cells (Figure 2B).
Albumin secretion in differentiated UCMSCs
(z = 3.75)
(z = 3.28)
(z = 3.45)
Periodic acid-Schiff staining also showed that the UCMSCs subjected to the hepatogenic differentiation protocol were able to store glycogen after 14 days of culture, while the undifferentiated UCMSCs did not (Figure 2C).
Urea measured after 28 days of culture in hepatogenic medium showed that cells were able to metabolize ammonia [1.222 (1.134; 1.304) mM/104cells/day]. Undifferentiated cells produced lower levels of urea [0.284 (0.273; 0.328) mM/104cells/day]. Mann Whitney-U test showed that this difference was statistically significant (*p = 4.246x10-5).
Characterization of UCMSCs after 28 days of culture
In vitroUCMSC hepatogenic differentiation on Matrigel™
qPCR showed that expression of hepatic markers AFP, albumin and MTP, with the exception of TDO messenger, was modulated during the differentiation process at each time point.
AFP mRNA expression in UCMSCs cultured in differentiating medium was significantly lower than in the corresponding control cells, but it remained stable up to 28 days (Table 1).
Although initially down-regulated, albumin mRNA expression showed a rising trend, but this up-regulation was only significant at 28th day of differentiation. Cuzick’s trend test showed that up-regulation were time-dependent (¥p = 0.0065 z = 2.72).
MTP mRNA expression was significantly up-regulated from the 21st day onwards in comparison with the control values and this increase was time-dependent (¥p = 0.0122 z = 2.51). TDO mRNA expression remained stable compared to control cells and also during the differentiating process (Table 1).
As previously obtained with the untreated plastic support, immunocytochemistry analyses showed that albumin protein was only expressed by the differentiated cells after 14 days and no AFP expression was detectable in the differentiated or in control cells (Figure 3B).
Functional tests demonstrated that albumin was secreted in the supernatants of differentiated UCMSCs cultures in a time-dependent manner (¥p = 0.0010; z = 3.29), reaching levels comparable with those of the differentiated cells seeded on untreated plastic support (Table 2).
No glycogen storage was observed in the undifferentiated cells, whereas the UCMSCs cultured with differentiating medium were positive to PAS staining already after 14 days (Figure 3C).
Urea measured after 28 days of culture in hepatogenic medium showed that cells were able to metabolize ammonia in a comparable fashion with those measured on cells differentiated on untreated plastic support [1.197 (1.161; 1.240) mM/104cells/day]. Undifferentiated cells produced lower levels of urea [0.292 (0.257; 0.309) mM/104cells/day]. Mann Whitney-U test showed that this difference was statistically significant (*p = 2.538x10-5).
In vitroUCMSCs hepatogenic differentiation on hLAM
In order to verify the differentiating capability of UCMSCs in vitro on a more adapt biological support, we seeded the cells on human liver acellular matrix.
Preliminary histochemical and scansion electronic microscopy (SEM) analyses showed that the matrix sections obtained from human liver were completely decellularized after a single treatment cycle. Masson’s trichrome staining also confirms that the matrix retained its fibrillary components after the treatment (data not shown).
qPCR analysis showed that the AFP and albumin mRNA expression in UCMSCs seeded on hLAM either in proliferative and differentiating medium remained stable during the time. MTP and TDO mRNA were never expressed in either proliferative or differentiating conditions (Table 1).
mRNA expression and albumin secretion at each time-point
A) Kruskal-Wallis test
B) Kruskal Wallis test followed by Bonferroni correction
Petri dishes vs. hLAM
p < 0.0001
p < 0.0001
p < 0.0001
MatrigelTM vs. hLAM
p < 0.0001
p < 0.0001
p < 0.0001
Petri dishes vs. MatrigelTM
p < 0.0001
p < 0.0001
p < 0.0001
p < 0.0001
Comparison between supports: untreated plastic is most suitable
To assess the differences between the three supports considered in our study we performed a multiple comparison of the expression levels of each marker analyzed and the albumin secretion at each time point. This analysis clearly suggested a difference between the supports (Table 4A). The Kruskal-Wallis followed by Bonferroni correction demonstrated that hLAM was the most unsuitable support for the purpose of UCMSCs differentiation. In fact, the pairwise comparison showed that the AFP mRNA down-regulation and albumin mRNA up-regulation were more marked in petri dishes. Furthermore, the albumin secretion was unequivocally stronger when the cells were seeded on petri dishes or on MatrigelTM rather than seeded on hLAM. In addition, the absence of any MTP and TDO mRNA, suggest that the UCMSCs cultured on hLAM failed to reach the hepatocyte-like phenotype (Table 4).
Pairwise comparison between petri dishes and Matrigel™ brings out that the AFP mRNA down-regulation was more pronounced on petri dishes and reaching significant levels at 28th day of differentiation (§p = 0.0118).
Finally, MTP mRNA up-regulation – which was always time-dependent - seemed to be more marked in the cells seeded on untreated plastic support (¥p = 0.0021 vs. ¥p = 0.0122). The multiple comparison followed by Bonferroni correction showed that MTP mRNA induction after 14 and 28 days was significantly greater on petri dishes. Interestingly, TDO mRNA up-regulation was verified exclusively when the cells were seeded on untreated plastic support.
Transplanted UCMSCs accelerate the recovery after CCl4-induced liver injury
In order to evaluate the therapeutic potential of the UCMSCs in an acute liver damage we transplanted them into CCl4-treated mice. Considering their hepatocyte-like phenotype and their capability to differentiate in hepatocyte-like cells we transplanted undifferentiated UCMSCs.
UCMSCs transplantation in CCl4-treated mice reduced the amount of inflammatory cells at day 5 and the necrotic areas were about 17.2% of parenchyma. Necrotic areas, inflammatory recruited cells and activated mega macrophages were completely absent after 8 days (Figures 4 and 5). Therefore histological pattern developed characteristics similar to those of control healthy mice parenchyma (Additional file 2: Figure S2).
Transplanted UCMSCs were able to reduce liver inflammation and to inhibit stellate cell and myofibroblasts activation
On the contrary, in UCMSCs transplanted mice there were not positive cells for alpha-SMA except vascular smooth muscle cells and few desmin and nestin positive cells were visible (Figure 8).
UCMSCs influenced liver antioxidant enzyme activity
Studies on stem cells and on their potential sources have been intensified in recent years, given the promise of their clinical application, especially in regenerative medicine .
Ethical problems regarding the use of human embryos  and the neoplastic risks after their in vivo use  have led to adult stem cells being considered a more acceptable source. Bone marrow is a good source of adult stem cells but the decrease in number of stem cells available with the donor age and invasive procedure required to obtain the cells are the major problems for their utilization [12, 13]. Research has consequently turned towards finding alternative sources of MSCs, such as adipose tissue  and foetal-derived tissues. Placenta, amniotic fluid and umbilical cord indeed seem to contain undifferentiated cells, due to their embryonal origin [15–18].
In this study we characterized a novel MSCs population obtained from human UC (UCMSCs) and we have induced their differentiation towards hepatic lineages in vitro seeking the best cell support for this purpose.
Phenotypic analysis showed a profile compatible with MSCs and the simultaneous high expression of CD166, CD105 and CD73 demonstrated that our cells were a novel MSCs population.
We found that these UCMSCs constitutively express mRNA coding for specific hepatic markers such as AFP, albumin and TDO. Then, for the first time, we unexpectedly demonstrated that undifferentiated UCMSCs constitutively expressed mRNA coding for MTP, a transfer protein localized in the endoplasmic reticulum of mature hepatocytes and enterocytes; this protein catalyses the transport of triglycerides, cholesteryl esters and phospholipids .
We believe that these findings reinforce the conviction that UCMSCs have a strong potential for differentiating into hepatic-lineage cells in vitro.
In fact, when UCMSCs were stimulated to differentiate towards a hepatic lineage, reached a hepatocyte-like phenotype, amazingly, when seeded on a simple untreated plastic support.
Further confirmation comes from flow cytometric analysis on differentiated cells that demonstrated a conspicuous drop in almost all the typical MSCs markers, such as CD73, CD90, and CD105. The only exception was CD166 expression that was spontaneously lost in UCMSCs cultured in proliferative medium while was maintained when the cells were submitted to differentiation protocol. Interestingly, CD29, another typical MSC marker, was expressed higher in differentiated UCMSCs (79,4%) reaching a level comparable with human hepatocytes .
The morphological features, loss of MSC phenotype, gene expression changes, immunocytochemical staining, albumin secretion, urea production and glycogen storage, all suggested that these cells can grow and differentiate into functional hepatocyte-like cells without any biological support, whereas cells seeded on 3D-supports showed a minor (MatrigelTM) or negligible (hLAM) differentiation capacity. Indeed, the data that unquestionably confirm our assertions was on TDO mRNA modulation that was found exclusively when the cells were differentiated on petri dishes. Our data would indicate that hLAM is not a suitable support for cell growth and hepatic differentiation considering that the cells lost mRNA coding for MTP and TDO after 7 days of culture and the supplementation with hepatogenic differentiating factors did not influence the basal AFP and albumin mRNA levels during 28 days of stimulation. These findings suggest that, in our experimental conditions, the coating afforded no advantage over an untreated plastic support for the purpose of UCMSCs hepatic differentiation; on the contrary, the simplest support seems to be the most suitable for this aim.
Taken together these results demonstrated that this novel population is an ideal candidate for liver disease treatment by cell therapy.
Mesenchymal stem cell transplantation has been explored as a new clinical approach to repair injured tissue. A growing number of studies have highlighted two important aspects of MSC therapy: MSCs can modulate T-cell mediated immunological responses [9, 34] and following systemic administration these cells home to sites of ischemia or injury, as was demonstrated in lung , heart , kidney  and liver . Several different MSC sources have been evaluated for cell therapy in chronic and acute liver diseases, such as bone marrow , amniotic fluid  and human umbilical cord blood [38, 39]. On the contrary, UCMSCs have been exclusively considered for therapy in chronic liver diseases, such as fibrosis and cirrhosis, and so far liver UCMSCs transplantation in situ has been the unique administration route [40, 41].
It has been documented that multipotent MSCs synthesize a wide variety of growth factors and cytokines, exerting a paracrine effect on local cellular dynamics . Such trophic effects could be irrespective of direct differentiation of transplanted cells into lineages of the respective tissues as demonstrated in an ischemic acute renal failure model . The hepatogenic potential and immunomodulatory activity of UCMSCs were further investigated in this study employing systemic transplantation in a murine model of acute liver injury induced by a single administration of carbon tetrachloride, able to induce severe hepatic damage by generation of oxidative stress and activation of immune cells . In fact, so far the possibility of using human UCMSCs to repair acute liver damage has not been evaluated and they have been transplanted via systemic administration by our group for the first time. MSCs were found to be very resistant to ROS and induced a faster reduction of oxidative stress in recipient mice . For these reasons, we decided to transplant undifferentiated UCMSCs rather than pre-differentiated cells.
We transplanted UCMSCs after 24 hours from the damage, when 40% of hepatic parenchyma was necrotic. Cells were recruited in the injured tissue and then they were able to engraft the liver (cells did not reach the liver when transplanted in healthy mice) and to regulate the inflammatory process. In fact in transplanted mice as soon as 5 days after the CCl4 injection, the inflammatory process was clearly attenuated, showing a moderated infiltrate, a lower CD68 positivity, a lower pro-inflammatory cytokines expression (primarily TNF-alpha and TGF-beta 1) and a higher level of IL-10 gene expression compared to the CCl4 alone group.
At day 8 histological analysis of liver of CCl4 treated mice showed the presence of cellular clusters that we identified as mega macrophages and activated Kupffer cells. In transplanted mice it was possible to identify exclusively Kupffer cells only at day 5, whereas after 8 days this activation phase was completely terminated. These findings suggest that transplanted UCMSCs have anti-inflammatory properties or are able to accelerate the kinetic of inflammatory process, leading to liver recovery in a shorter time.
During the pro-inflammatory process induced by CCl4, jointly with Kupffer cells, stellate cells play a pivotal role. Immunofluorescence analysis showed that in CCl4-treated mice there was a conspicuous number of alpha-SMA positive cells. The most of these cells were identified as activated stellate cells since they co-expressed desmin and nestin. Alpha-SMA single positive cells were another subpopulation of liver myofibroblasts. Following cells transplantation there was no activation of stellate cells, therefore, the anti-inflammatory activity of UCMSCs was directed not only towards inflammatory cells, including Kupffer cells but also against stellate cells and myofibroblasts. TGF-beta 1 down-regulation in transplanted mice confirmed our hypothesis.
The liver has several antioxidant enzymatic systems such as superoxide dismutase, catalase and glutathione peroxidase that play a fundamental role during physiological and pathological ROS mediated oxidative stress. We evaluated whether UCMSCs were able to ameliorate hepatic damage also influencing the antioxidant systems, measured by catalase activity within the liver. In CCl4 treated mice livers the activity of catalase was significantly reduced compared with control groups. The enzyme was probably degraded or saturated to block CCl4-induced massive free radical production. In presence of mesenchymal stem cells catalase activity was higher after 8 days compared to CCl4 treated mice. These finding suggested that UCMSCs reduced catalase consumption, confirming that oxidative damage in transplanted mice was nearly resolved.
Our experiments also showed that catalase activity measured in the liver of UCMSCs transplanted mice without any CCl4 induced damage (MSCs group) was higher compared to PBS group. Therefore we speculate that UCMSCs contribute to scavenging activity against radicals by stimulating the activity of catalase, one of the biological defence system of the liver.
In vivo experiments showed that unquestionably UCMSCs were able to induce total liver recovery acting as an adjuvant or modulating the physiologic defence systems. There is not a direct relationship between the number of cells that are found in the liver and the amazing results in transplanted mice. It was demonstrated that stem cells act through a dual mechanism: cell-to cell contact and modulation mediated by soluble factors produced by cells themselves. Our hypothesis is that cells have immunomodulatory activity both at local level and before homing to damaged tissue. It is possible that cells are able to act also during their permanence in the bloodstream and completing their action upon reaching the liver. Increased catalase activity measured in MSCs group demonstrated that cells were able to influence hepatic antioxidant environment without liver engraftment.
Our results look promising for future application of UCMSCs in regenerative medicine and clinical practice. The chances of using such cells in this field are increased by the simplicity and reproducibility with which a significant number of cells can be obtained, by the homogeneous features between different cellular preparations that need not to undergo any sorting, by the absence of immunogenicity (because UCMSCs are HLA-DR-negative) and, most importantly, by the fact that they carry no legal or ethical implications. Cell transplantation is a practical procedure compared with organ transplantation. It can be performed with much less risk to the patient and much reduced cost for the healthcare system. Furthermore, given the little invasiveness of systemic administration, this method could be also applied to patients who are severely ill and would not be able to tolerate organ transplantation. We are aware that the conveyance of our results into clinical practice would need to be considered with caution because more information is needed on these cells’ behavior in vivo before any clinical applications can be hypothesized, but the potential shown by our UCMSCs is undeniably fascinating and holds promise.
MSCs isolation from human umbilical cord
Umbilical cords from full-term deliveries were obtained with the written informed consent of the mothers at the Obstetrics and Gynecology Unit of Cittadella Hospital and processed within 24 hours. They were collected in accordance with the requirements of the local ethical committee. The umbilical vessels were manually removed and the jelly was minced to obtain small fragments, which were plated in 100-mm petri dishes and cultured with a proliferative medium [high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM) with stable L-glutamine (Euroclone S.p.a., Milan, Italy), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen Life Technologies, Carlsbad, USA), and 20% FBS specific for MSC cultures (Stem Cell Technologies Inc., USA)]. To allow the cells to migrate from the tissue fragments, the medium was first replaced after 5–7 days, and subsequently twice a week.
The UCMSCs were analyzed by flow cytometry for the expression of the following antigens: CD14, CD29, CD34, CD45, CD71, CD73, CD90, CD105, CD166, c-kit and HLA-DR (Human Leukocyte Antigen-DR, a MHC class II cell surface receptor). As a negative control, isotype control antibodies conjugated with FITC and RPE (Santa Cruz Biotechnology, California) were used. The cytofluorimetric analysis was performed with the MoFlo High-Speed Cell Sorter (DAKO-Beckman Coulter, USA) and the data were analyzed using Summit 4.3 software (DAKO-Beckman Coulter, USA).
Mesenchymal stem cell features of UCMSCs isolated were investigated by adipogenic and osteogenic differentiation assay and their foetal origin was verified through SRY (sex-determining region Y) gene analysis, as previously described by our group .
Human Liver Acellular Matrix (hLAM)
The hLAM was analyzed by Masson trichrome staining (Bio-Optica, Milan, Italy) according to the manufacturer’s instructions and by scanning electron microscopy (SEM) to assess its structure and fibrillary components.
In vitrohepatogenic differentiation on the three different supports
UCMSCs seeded on untreated plastic supports were cultured with hepatogenic differentiating medium consisting of Iscove’s Modified Dulbecco’s Medium (IMDM) (Euroclone S.p.a., Milan, Italy) supplemented with 15% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin, 1% L-glutamine, 10-7 M dexamethasone, 10 ng/ml oncostatin M (Sigma-Aldrich, St. Louis, MO), 10 mg/ml ITS supplement (Roche Applied Sciences, Italy), 20 ng/ml hepatocyte growth factor (HGF) and 100 ng/ml fibroblast growth factor (FGF-4) (Pepro Tech EC, London, UK). Cells cultured in proliferative medium were used as a control.
The media were changed twice a week and the cells were collected at days 7, 14, 21 and 28 after induction. Immunocytochemical analyses were performed for the hepatic marker AFP and albumin. The cultures were fixed with 2% paraformaldehyde solution for 20 min at 4 °C and washed in PBS. Immunocytochemical staining was done as previously reported . UCMSCs were seeded on plastic supports coated with MatrigelTM (BD Biosciences Bedford, MA, USA), diluted 1:10 with DMEM, and cultured for up to 28 days, then stained as described above, or on hLAM seeded on a 24-well plate coated with hLAM and cultured as described previously up to 28 days.
RNA isolated from UCMSCs at each time point was reverse-transcribed and amplified with the following primers: 5′-TGACTCCAGTAAACCCTGGT-3′ and 5-AGAAATCTGCAATGACAGCC-3′ for AFP; 5′-ATCAAGAAACAAACTGCACT-3′ and 5′-GCAGGTCTCCTTATCGTCAG-3′ for albumin; 5′-CGTTCGGCATCTACTTACAGC-3′ and 5′-GTTCTCCTCCCCCTCGTCAG-3′ for MTP; 5′-GGAGAAGAAAATGAACTGCTAC-3′ and 5′-GGCTCTAAACCTGGAGT-3′ for TDO; 5′-AGCAACAGGGTGGTGGAC-3′ and 5′-GTGTGGTGGGGGACTGAG-3′ for GAPDH.
RNA isolated from mice liver was reverse-transcribed and amplified with the following primers: 5’-TCTTCTCATTCCTGCTTGTGG-3’ and 5’-GGTCTGGGCCATAGAACTGA-3’ for TNF-alpha; 5′-AAGAGAAGTGTGGCGAGGAG-3′ and 5′-CAGTTTTGTGGGGTTTTTGC-3′ for IL-5; 5’-TGGAGCAACATGTGGAACTC-3’ and 5’-GTCAGCAGCCGGTTACCA-3’ for TGF-beta 1; 5’-CCAGTTTTACCTGGTAGAAGTGATG-3’ and 5’-TTTTCACAGGGGAGAAATCG-3’ for IL-10; 5′-GATTACTGCTCTGGCTCCTA-3′ and 5′-TCGTACTCCTGCTTGCTGAT-3′ for beta-actin.
After amplification, the data were analyzed using the second-derivative algorithm. For each sample, the quantity of specific hepatic marker mRNA was expressed as n-fold the normalized amount of mRNA from undifferentiated cells.
Albumin secretion in the culture medium was quantified by ELISA according to the manufacturer’s instructions (Alpha Diagnostic Intl. Texas, USA).
Urea concentration was quantified in supernatants collected from differentiated and undifferentiated cells after 28 days. 24 hours before collection of supernatants NH4Cl 0.3 mM was added to the medium. Urea concentration was measured by a colorimetric assay, according to the manufacturer’s instructions (Gentaur, Brussels, Belgium).
Periodic Acid-Schiff Staining (PAS) and PAS-Diastase (PAS-D)
Cells were fixed using 4% paraformaldehyde for 10 minutes. After oxidizing in 0.5% periodic acid (Merck, NJ USA) for 5 minutes, they were treated with Schiff’s reagent (Merck, NJ USA) for 15 minutes, then rinsed in water for 10 minutes and counterstained with haematoxylin.
For PAS-D the tissue-slides were incubated with diastase solution (0.001 g/L in distilled water) in waterbath. After washing, PAS stain standard procedure was performed as described above.
CCl4-induced liver injury and UCMSCs transplantation
Mice were divided randomly into five study groups with 6 animals per group. For acute CCl4-induced liver injury eight- week-old male Balb/c mice were injected (i.p.) with a single dose of CCl4 (0.75 ml/kg body weight of CCl4 dissolved in olive oil). The control mice received the same isovolumetric dose of olive oil as i.p. injections.
106 undifferentiated UCMSCs at passage 2 resuspended in 150 μl of PBS were injected via tail vein 24 hours after CCl4 injection. Control animals received the same volume of PBS or UCMSCs alone. Animal care was in accordance with our institutional guidelines.
Animals were sacrificed at day 5 and day 8 after CCl4 injection; liver samples were collected and right lobe was fixed in 4% formalin for 24 hours and embedded in paraffin. The others lobes were stored at −80 °C until assayed.
Four-micrometer-thick liver sections were deparaffinized and rehydrated. For histological examination the slides were stained with haematoxylin and eosin. Morphometric analysis was performed measuring the percentage of necrotic areas in 6 fields versus total section area. For immunofluorescence analysis antigen retrieval was performed by microwave-heating method in 0.01 mol/L citrate buffer (pH 6). Slides were preincubated in goat serum (Sigma-Aldrich, St. Louis, MO) at 1:25 dilution in PBS for 30 minutes and then were incubated in primary antibody (monoclonal anti-alpha-SMA; monoclonal anti-human albumin, polyclonal anti-desmin and polyclonal anti-nestin, Sigma-Aldrich, St. Louis, MO) at a dilution from 1:100 to 1:2000 for 1 hour. The secondary antibody was a FITC-conjugated anti-mouse (Sigma-Aldrich, St. Louis, MO) and rhodamine-conjugated anti-rabbit (Santa Cruz Biotech., CA) at a dilution of 1:160 for 45 minutes. The section was counterstained with the nuclear marker DAPI (Vector Laboratories). Sections were imaged on Leica SP5 Confocal Microscope.
The number of positive cells was counted in 15 random fields (100x) and expressed as cells for field.
For immunohistochemistry sections were stained with anti-mouse CD68 antibody (Sigma-Aldrich, St. Louis, MO). Detection was performed after incubating the sections with horseradish peroxidase–conjugated anti-mouse antibody. Peroxidase activity was revealed by 5-minute exposure to diaminobenzidine (Sigma-Aldrich, St. Louis, MO). After washing, preparations were counterstained with Mayer’s haematoxylin for 10 minutes and mounted for analysis.
Determination of catalase activity
Liver homogenates were prepared in phosphate buffer containing 0.5% triton (pH = 7.4). Samples were centrifuged at 12,000 x g for 30 min at 4 °C. The supernatant was collected and protein concentration was estimated by BCATM protein assay kit (Pierce Company, Rockford, IL USA).
Catalase activity was determined by measuring the exponential disappearance of H2O2 at 240 nm and expressed in units/mg of protein as described by Aebi .
Data are given as median (Quartile 1; Quartile 3). The non-parametric Mann–Whitney U-test was used to assess differences between differentiated and control cells; time trends were evaluated using the Cuzick’s test based on Wilcoxon’s approach. Kruskal-Wallis test was used for multiple comparisons followed by the post hoc Bonferroni correction.
A p-value lower than 0.05 was assumed to indicate a significant difference (Bonferroni-adjusted at 0.016). Data analyses were performed with SPSS and StatsDirect.
This study was supported by grants from COPEV (Comitato per la Prevenzione dell'Epatite Virale)" to PBu, grant from University of Padua (Progetto di ateneo 2008) to FPR and grant from “Regional Centre on Cell Therapy for the Metabolic Liver Diseases, Regione Veneto, Italy, to PBu.
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